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Pt246 pras40

Manufactured by Thermo Fisher Scientific
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The PT246-PRAS40 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It serves as a tool for scientific research and analysis. The core function of this product is to provide a platform for the measurement and analysis of PRAS40 protein levels. No further details or interpretation on the intended use of this product can be provided in an unbiased and factual manner.

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Lab products found in correlation

Magpix, by Bio-Rad (4 mentions) Ps9 gsk 3β, by Thermo Fisher Scientific (4 mentions) Gsk 3β, by Thermo Fisher Scientific (4 mentions)

4 protocols using pt246 pras40

1

Multiplex ELISA Profiling of Insulin Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
We used bead-based multiplex ELISAs to measure immunoreactivity to the
insulin receptor (IR), IGF-1 receptor (IGF-1R), IRS-1, Akt, proline-rich Akt
substrate of 40 kDa (PRAS40), ribosomal protein S6 kinase (p70S6K), and glycogen
synthase kinase 3β (GSK-3β), and pYpY1162/1163-IR,
pYpY1135/1136-IGF-1R, pS312-IRS-1,
pS473-Akt, pT246-PRAS40, pTpS421/424-p70S6K,
and pS9-GSK-3β (Invitrogen, Carlsbad, CA). Samples (100
μg protein) were incubated with the beads, and captured antigens were
detected with secondary antibodies and phycoerythrin-conjugated anti-rabbit IgG
[33 (link)]. Plates were
read in a MAGPIX (Bio-Rad, Hercules, CA).
Andreani T., Tong M., Gundogan F., Silbermann E, & de la Monte S.M. (2016). Differential Effects of 3rd Trimester-Equivalent Binge Ethanol and Tobacco-Specific Nitrosamine Ketone Exposures on Brain Insulin Signaling in Adolescence. Journal of diabetes and related disorders, 1(1), 105.
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2

Multiplex ELISA Assay for Insulin Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used bead-based multiplex ELISAs to measure immunoreactivity to the insulin receptor (IR), IGF-1 receptor (IGF-1R), IRS-1, Akt, proline-rich Akt substrate of 40 kDa (PRAS40), ribosomal protein S6 kinase (p70S6K), and glycogen synthase kinase 3β (GSK-3β), and pYpY1162/1163-IR, pYpY1135/1136-IGF-1R, pS312-IRS-1, pS473-Akt, pT246-PRAS40, pTpS421/424-p70S6K, and pS9-GSK-3β (Invitrogen, Carlsbad, CA). Samples (50 μg protein) were incubated with the beads, and captured antigens were detected with secondary antibodies and phycoerythrin-conjugated anti-rabbit IgG. Plates were read in a MAGPIX (Bio-Rad, Hercules, CA).
de la Monte S.M., Tong M., Agarwal A, & Cadenas E. (2016). Tobacco Smoke-Induced Hepatic Injury with Steatosis, Inflammation, and Impairments in Insulin and Insulin-Like Growth Factor Signaling. Journal of clinical & experimental pathology, 6(2), 269.
+ Open protocol
+ Expand
3

Multiplex ELISA Profiling of Insulin Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
We used bead-based multiplex ELISAs to measure immunoreactivity to the
insulin receptor (IR), IGF-1 receptor (IGF-1R), IRS-1, Akt, proline-rich Akt
substrate of 40 kDa (PRAS40), ribosomal protein S6 kinase (p70S6K), and glycogen
synthase kinase 3β (GSK-3β), and pYpY1162/1163-IR,
pYpY1135/1136-IGF-1R, pS312-IRS-1,
pS473-Akt, pT246-PRAS40, pTpS421/424-p70S6K,
and pS9-GSK-3β (Invitrogen, Carlsbad, CA). Samples (100
μg protein) were incubated with the beads, and captured antigens were
detected with secondary antibodies and phycoerythrin-conjugated anti-rabbit IgG
[33 (link)]. Plates were
read in a MAGPIX (Bio-Rad, Hercules, CA).
Andreani T., Tong M., Gundogan F., Silbermann E, & de la Monte S.M. (2016). Differential Effects of 3rd Trimester-Equivalent Binge Ethanol and Tobacco-Specific Nitrosamine Ketone Exposures on Brain Insulin Signaling in Adolescence. Journal of diabetes and related disorders, 1(1), 105.
+ Open protocol
+ Expand
4

Multiplex ELISA Assay for Insulin Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used bead-based multiplex ELISAs to measure immunoreactivity to the insulin receptor (IR), IGF-1 receptor (IGF-1R), IRS-1, Akt, proline-rich Akt substrate of 40 kDa (PRAS40), ribosomal protein S6 kinase (p70S6K), and glycogen synthase kinase 3β (GSK-3β), and pYpY1162/1163-IR, pYpY1135/1136-IGF-1R, pS312-IRS-1, pS473-Akt, pT246-PRAS40, pTpS421/424-p70S6K, and pS9-GSK-3β (Invitrogen, Carlsbad, CA). Samples (50 μg protein) were incubated with the beads, and captured antigens were detected with secondary antibodies and phycoerythrin-conjugated anti-rabbit IgG. Plates were read in a MAGPIX (Bio-Rad, Hercules, CA).
de la Monte S.M., Tong M., Agarwal A, & Cadenas E. (2016). Tobacco Smoke-Induced Hepatic Injury with Steatosis, Inflammation, and Impairments in Insulin and Insulin-Like Growth Factor Signaling. Journal of clinical & experimental pathology, 6(2), 269.
+ Open protocol
+ Expand

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