Frozen fecal samples were vortexed with PBS to obtain a homogenous suspension. Samples were then centrifuged for 10 min at 9,500 g, at 4°C. Supernatants were collected and stored at −80°C until analysis. Lipocalin-2 levels were estimated in the supernatants using Duoset murine lipocalin-2 ELISA kit (R&D Systems, Minneapolis, MN). Calprotectin levels were estimated in the supernatants using the S100A9/Calprotectin mouse ELISA kit (Hycult Biotech, Plymouth Meeting, PA).37 Both were standardized to total protein, as determined using measured with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA).
S100a9 calprotectin mouse elisa kit
Manufactured by Hycult Biotech
The S100A9/Calprotectin mouse ELISA kit is a laboratory equipment product manufactured by Hycult Biotech. It is designed to detect and quantify the levels of the S100A9 protein, also known as Calprotectin, in mouse samples using the enzyme-linked immunosorbent assay (ELISA) technique.
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Lab products found in correlation
2 protocols using s100a9 calprotectin mouse elisa kit
1
Analyzing Fecal Biomarkers: Lipocalin-2 and Calprotectin
2
Analyzing Fecal Biomarkers: Lipocalin-2 and Calprotectin
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Frozen fecal samples were vortexed with PBS to obtain a homogenous suspension. Samples were then centrifuged for 10 min at 9,500 g, at 4°C. Supernatants were collected and stored at −80°C until analysis. Lipocalin-2 levels were estimated in the supernatants using Duoset murine lipocalin-2 ELISA kit (R&D Systems, Minneapolis, MN). Calprotectin levels were estimated in the supernatants using the S100A9/Calprotectin mouse ELISA kit (Hycult Biotech, Plymouth Meeting, PA).37 Both were standardized to total protein, as determined using measured with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA).
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