U118mg cells

Human colorectal adenocarcinoma LS174T and human glioma U118MG cells were purchased from ATCC (Manassas, VA). Human pancreatic carcinoma HS766T cells were kindly provided by Dr PG Oliver (University of Alabama at Birmingham, Birmingham, AL). Human glioma U118-hCAR cells expressing hCAR were kindly provided by Dr JT Douglas (University of Alabama at Birmingham). For propagation of our vector we used HEK293 cells, purchased from Microbix Biosystems (Mississauga, Ontario, Canada), and 293F28 cells expressing wild-type Ad5 fiber protein, which have been described previously.^{37 (link)} All abovementioned cell lines were cultured in DMEM/F12 (Mediatech, Nerndon, VA) medium supplemented with 10% FBS, 100 IU/ml penicillin and 100 µg/ml streptomycin.
The murine colon adenocarcinoma MC38 and MC38CEA cells have been described previously.^{38 (link)} MC38CEA cells expressing hCEA were generated by retroviral transduction with hCEA cDNA. MC38 and MC38CEA cells were provided by Dr HR Herschman (University of California, Los Angeles, CA) and cultured in DMEM medium supplemented with 10% FBS, 0.1 mmol/l NaPyruvate, 100 IU/ml penicillin, and 100 µg/ml streptomycin.
Immortalized primary human liver THLE-3 cells were purchased from ATCC and cultured in accordance with vendor instructions.