Indole acetic acid (IAA) production of PSMs was determined according to the method of Gordon and Weber (1951) (link) with some modifications. The strain was incubated in a potato dextrose agar (PDA) medium for fungi and a Luria-Bertan (LB) medium for bacteria supplemented with 2mg•mL -1 of tryptophan at 30 °C for 6 days. Uninoculated PDA or LB liquid medium was used as a control. Each experiment was conducted in three triplicates. After that, the fermentation broth was centrifuged at 10000 rpm for 10 min. Then, 2 mL of the supernatant was mixed with 4 mL of Salkowski solution including 35% of HClO4 and 0.5 mol•L -1 FeCl3. The mixture was incubated in the dark at 40 °C for 30 min. Finally, IAA was measured by a spectrophotometric method (UV-6100; Shanghai Metash Instruments Co., Ltd., Shanghai, China) at 530 nm and was calculated from the standard curve of pure IAA (Asghar et al. 2002) (link).
Uv 6100
Manufactured by Metash
Sourced in China
The UV-6100 is a compact and versatile ultraviolet-visible (UV-Vis) spectrophotometer. It is designed to measure the absorbance or transmittance of samples across a range of ultraviolet and visible light wavelengths.
Automatically generated - may contain errors
14 protocols using uv 6100
1
Quantification of Indole Acetic Acid in Microbes
2
Measurement of Myofibrillar Fragmentation Index
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MFI was determined by modification of the method by Hou et al. [25 (link)]. Two gram samples were homogenized with a homogenizer (FJ200-S, Shanghai Specimen Model Co., Shanghai, China) at 10,000 g for 60 s (3 × 20 s with a 60 s break between bursts) at 4 ± 2 °C in 20 mL ice-cold buffer (100 mM KCl, 20 mM K2HPO4, 1 mM EGTA, 1 mM MgCl2, and 1 mM NaN3, pH 7.0). The homogenates were centrifuged using a LG10-24A model centrifuge (Peking Medical centrifuge Factory, Beijing, China) at 3000 g for 15 min at 4 °C and the supernatant was discarded. The pellets were homogenized in 20 mL of homogenizing buffer and centrifuged and the supernatant was discarded again. The resulting pellets were then resuspended in 5 mL of homogenizing buffer and filtered through a polyethylene strainer (200-mesh) to remove the fat and connective tissue. Then, 5 mL buffer was used to promote the passage of myofibrils through the strainer. The protein concentration of suspension was determined by the biuret method [26 (link)]. The protein concentration was diluted to 0.5 mg/mL and measured spectrophotometrically at 540 nm (UV 6100, Metash, Shanghai, China). MFI was calculated by multiplying A540 by 200.
3
Characterization of Embedded Metal Mesh in Flexible Transparent Electrodes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical images of the metal mesh were captured through an optical microscope (DSX510, OLYMPUS, Japan; Phenix MC‐D500U(C), China). The microstructure and cross‐section of the metal mesh was characterized via field‐emission SEM (MERLIN Compact, Zeiss, Germany). The printing process of the embedded metal mesh was captured by a high‐speed camera (i‐SPEED221, IX Cameras, UK). The embedded metal mesh was conductivity‐treated in a vacuum drying oven (DHG‐903385‐III, Shanghai Shengke Instrument Equipment Co., Ltd., China). The optical transmittance of the metal mesh was measured with a UV–vis spectrophotometer (UV‐6100, Metash, China). The heating performance of FTE was captured by infrared thermal imaging (TG165, FLIR Systems. USA). The Rs of the FTE is measured by a milliohmmeter (AT516, Applent Instruments Co., Ltd., China). The damp heat test of embedded metal mesh was measured by constant temperature and humidity test chamber (Dongguan Seth Testing Equipment Co., Ltd., China). The bending fatigue test was completed via a self‐developed test system.
4
Quantifying Total Phenolic Content
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total phenolic content (TPC) was measured according to the described method [54 (link)]. Sample solution (1.0 mL) was mixed with the Folin–Ciocalteu reagent (5.0 mL; 0.1 mmol/L) and then incubated at room temperature for 5 min. After, a sodium carbonate solution (4.0 mL; 75 g/L) was added, and the mixture was incubated at room temperature for 30 min in darkness. A gallic acid solution was used as the standard and the absorbance was measured at 765 nm by the spectrophotometer (UV-6100; Shanghai Metash Instrument Co., Ltd., Shanghai, China). TPC was expressed as mg gallic acid equivalents per gram of extract (mg GAE/g extract). All measurements were performed in triplicate.
5
Characterization of Protein-Carbohydrate Conjugates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The formation of F30, F30-10, or F10-dextran conjugates was confirmed by measuring the amount of Amadori compounds and browning degree and analyzing the changes in the protein composition using the electrophoretic analysis mentioned above.
The amounts of Amadori compounds and browning degree were determined according to a method proposed by Wang and Ismail [12 (link)]. The samples were suspended in 0.01 mol/L pH 7.2 phosphate buffer solution (PBS) at a protein concentration of 2 mg/mL and then centrifuged at 9570× g for 10 min. The absorbance values of the supernatants at 304 nm and 420 nm (UV-visible spectrophotometer, UV-6100, Metash Instruments Co., Ltd., Shanghai, China) were defined as indications of Amadori compounds and the browning degree, respectively.
The method used to complete the electrophoretic analysis of conjugates was the same as that mentioned above, except that the separation of F10 and F10-dextran conjugate was performed using a 15% separating gel.
The amounts of Amadori compounds and browning degree were determined according to a method proposed by Wang and Ismail [12 (link)]. The samples were suspended in 0.01 mol/L pH 7.2 phosphate buffer solution (PBS) at a protein concentration of 2 mg/mL and then centrifuged at 9570× g for 10 min. The absorbance values of the supernatants at 304 nm and 420 nm (UV-visible spectrophotometer, UV-6100, Metash Instruments Co., Ltd., Shanghai, China) were defined as indications of Amadori compounds and the browning degree, respectively.
The method used to complete the electrophoretic analysis of conjugates was the same as that mentioned above, except that the separation of F10 and F10-dextran conjugate was performed using a 15% separating gel.
6
Isolation and Screening of IAA-Producing Bacteria
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The adhering soil on root systems was washed with sterile water, and the surfaces of roots were treated with NaClO (5%, w/v) for 3 min, following by hydrogen peroxide solution (3%, w/v) for 3 min. Then, the roots were washed with sterile water. The suspension of the root power was incubated on LB medium for 48 h at 30 °C to screen for endophytic bacteria. The suspension of rhizosphere soil was incubated on LB medium for 48 h at 30 °C to isolate rhizosphere bacteria. The IAA-producing capability of microbes was initially screened according to the method of Gordon and Weber with some modifications.16 (link) The obtained bacterial strains were incubated in LB liquid medium supplemented with or without l -tryptophan (l -Trp) at 37 °C and 30 °C for 3 days. Each experiment was conducted in triplicate. After that, the fermentation broth was centrifuged at 10 000 rpm for 10 min. Then, 2 mL of the supernatant was combined with 4 mL of Salkowski reagent (50 mL of 35% HClO4 and 1 mL of 0.5 M FeCl3). The solution was incubated in darkness for 30 min at 40 °C. The absorbance of IAA was measured at 530 nm using a UV-Vis spectrophotometer (UV-6100, Metash, Shanghai, China).
7
Leaf Pigment Content Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In each treatment, six healthy and fully expanded leaves were chosen to measure pigment content. Chlorophylls (Chl) and carotenoids (Car) content was extracted with 80% acetone, and absorbances (A) at 470, 647, and 663 nm were recorded with a spectrophotometer (UV-6100, Shanghai Metash Instruments, China). The contents of total Chl and Car were determined according to Lichtenthaler (1987) .
8
Isolation and IAA Production of Soil Bacteria
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Soil samples were collected from the corn farm in the Jining of the Shandong province, P. R. China, which was stored in sealed and sterile bags at 4°C. All chemicals were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China).
Isolation of the strain and determination of IAA content 10 g of the soil sample was mixed with 90 mL of distilled sterile water, which was stirred at 30 °C and 150 rpm for 30 min. The resultant soil solution was diluted and plated on Luria-Bertan (LB) agar medium including NaCl (10 g), yeast extract (5 g), tryptone (10 g), agar (18 g). IAA content of the strains was determined according to the method of Gordon and Weber (1951) (link) with some modi cations. The strain was incubated in LB liquid medium supplemented with 0.2 mg/mL of L-tryptophan at 30 °C for 6 days.
Uninoculated LB liquid medium was used as a control. Each experiment was conducted in triplicate. After that, the fermentation broth was centrifuged at 10000 rpm for 10 min. 2 mL of supernatant was combined with 4 mL of Salkowski reagent (50 mL of 35% HClO 4 & 1 mL of 0.5 M FeCl 3 ), which was incubated in darkness for 30 min at 40 ℃. The absorbance of Indole-3-acetic acid (IAA) was measured at 530 nm using a UV-VIS spectrophotometer (UV-6100, Metash, China).
Isolation of the strain and determination of IAA content 10 g of the soil sample was mixed with 90 mL of distilled sterile water, which was stirred at 30 °C and 150 rpm for 30 min. The resultant soil solution was diluted and plated on Luria-Bertan (LB) agar medium including NaCl (10 g), yeast extract (5 g), tryptone (10 g), agar (18 g). IAA content of the strains was determined according to the method of Gordon and Weber (1951) (link) with some modi cations. The strain was incubated in LB liquid medium supplemented with 0.2 mg/mL of L-tryptophan at 30 °C for 6 days.
Uninoculated LB liquid medium was used as a control. Each experiment was conducted in triplicate. After that, the fermentation broth was centrifuged at 10000 rpm for 10 min. 2 mL of supernatant was combined with 4 mL of Salkowski reagent (50 mL of 35% HClO 4 & 1 mL of 0.5 M FeCl 3 ), which was incubated in darkness for 30 min at 40 ℃. The absorbance of Indole-3-acetic acid (IAA) was measured at 530 nm using a UV-VIS spectrophotometer (UV-6100, Metash, China).
9
Growth Curve and Protoplast Optimization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Our original QDW strain was purified by plate streaking and cultured in shake flasks. The OD600 value was measured every 2 h using an ultraviolet spectrophotometer (UV-6100, METASH, Shanghai, China) to obtain the growth curve of QDW within 24 h. The optimum time for preparing protoplasts of the strain was obtained by evaluating the growth curve of the strain.
10
Determination of Total Phenolic Content
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total phenolic content was determined using the described method [13 (link)]. Sample solution (1.0 mL) was mixed with the Folin–Ciocalteu reagent (5.0 mL; 0.1 mmol/L) and allowed to incubate for 5 min. A sodium carbonate solution (4.0 mL; 75 g/L) was added, and the mixture was vortexed and incubated at 25 °C for 30 min in darkness. A standard gallic acid solution was used and the absorbance was determined at 765 nm by spectrophotometer (UV-6100; Shanghai Metash Instrument Co., Ltd., Shanghai, China). Total phenolic content was expressed as mg gallic acid equivalents per gram of extract (mg GAE/g ext.). All measurements were performed in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!