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2 protocols using water lc ms grade

1

Antioxidant and Antimicrobial Evaluation

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Standard D-(-)-quinic acid, L-(-)-malic acid, citric acid, chlorogenic acid, Folin–Ciocalteu's phenol reagent, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) were obtained from Sigma-Aldrich (Saint Louis, MO, USA). Methanol (high performance liquid chromatography grade) acetonitrile (LC-MS reagent) and water (LC-MS grade) were purchased from RCI Labscan Limited (Bangkok, Thailand). Formic acid (analytical grade) was purchased from JT Baker (Philipsburg, NJ, USA). A nylon syringe filter 0.45 μm was bought from Lubitech Technologies Ltd. (Shanghai, China). Muller-Hinton agar (MHA) and Muller-Hinton broth (MHB) were purchased from Difco Laboratories, Inc. (Difco, Franklin Lakes, NJ, USA).
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2

Antioxidant Capacity Evaluation Protocol

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Trolox ((±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), DPPH (2,2-diphenyl-1-picryhydrazyl), TPTZ (2,4,6-Tri(2-pyridyl)-s-triazine), Iron(II) sulfate heptahydrate (FeSO4), Folin-Ciocalteu reagent, gallic acid (≥99% purity), sodium carbonate, methanol, ethanol, and tricholoacetic acid were bought from Merck (Darmstadt, Germany). Ferric chloride (FeCl3) was provided by Ajax Finechem (Seven Hills, Australia). Dulbecco’s modified Eagle’s medium (DMEM), MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide), and L-glutamine Sigma-Aldrich Chemicals (St Louis, MO, USA) was bought from Sigma-Aldrich Chemicals (St Louis, MO, USA). Fetal bovine serum (FBS) was bought from Gibco-BRL (Auckland, New Zealand). Water (LC-MS grade) was provided by RCI Labscan (Bangkok, Thailand).
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