Percol

Manufactured by GE Healthcare

Percol is a laboratory centrifuge designed to separate and isolate different components in a liquid sample. It operates by applying centrifugal force to the sample, causing the denser materials to separate from the less dense ones. The device is commonly used in various scientific and medical research applications to purify and concentrate samples for further analysis.

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Lab products found in correlation

Rpmi 1640, by Merck Group (1 mentions) Nycodenz, by Progen Biotechnik (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

3 protocols using percol

Isolation of High-Purity Neutrophils

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Neutrophils were isolated from the blood of healthy volunteers. About 20 mL blood was layered onto 10 mL 6% dextran (from leuconostoc spp, MR 45 000; Sigma-Aldrich) in sterile 0.9% saline and 20 mL phosphate-buffered saline (PBS). The solution was left to sediment for 45 min. The buffy coat layer was removed and centrifuged at 300 g for 5 min in 50 mL PBS, the leucocyte-rich layer was removed and resuspended in 55% Percol (GE Healthcare). A Percol gradient was prepared from 81% and 67% Percol and the resuspended leucocytes were layered on top (in 55% Percol). The Percol gradient was centrifuged for 30 min at 700 g. The neutrophil layer was removed and washed in PBS followed by centrifugation at 300 g for 10 min. Neutrophils were resuspended in 1 mL double-distilled H₂O for 30 s and resuspended in 20 mL PBS. Cells were centrifuged and resuspended in RPMI-1640 (Sigma-Aldrich). Isolated neutrophils had a purity of >98% (see online supplementary figure S1).

Williams A.E., José R.J., Mercer P.F., Brealey D., Parekh D., Thickett D.R., O'Kane C., McAuley D.F, & Chambers R.C. (2016). Evidence for chemokine synergy during neutrophil migration in ARDS. Thorax, 72(1), 66-73.

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Subcellular Fractionation of Weibel-Palade Bodies

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HUVECs were grown to confluency, and after 4 days, they were homogenized using a ball-bearing homogenizer (Isobiotec, Heidelberg, Germany) essentially as described previously.^{17 (link)} Subcellular fractions were obtained by density gradient ultracentrifugation using a Beckmann Optima LX-100 XP ultracentrifuge equipped with a Ti50.2 fixed angle rotor. Briefly, homogenates were fractionated by 2 subsequent Percol (GE Healthcare, Eindhoven, The Netherlands) density gradients followed by 1 Nycodenz (Progen Biotechnik, Heidelberg, Germany) density gradient.^{17 (link)} Percoll fractions and Nycodenz fractions containing the WPBs were identified by VWF ELISA.^{18 (link)} Selected fractions were analyzed by immunoblotting for syntaxin-3.

Schillemans M., Karampini E., van den Eshof B.L., Gangaev A., Hofman M., van Breevoort D., Meems H., Janssen H., Mulder A.A., Jost C.R., Escher J.C., Adam R., Carter T., Koster A.J., van den Biggelaar M., Voorberg J, & Bierings R. (2018). Weibel-Palade Body Localized Syntaxin-3 Modulates Von Willebrand Factor Secretion From Endothelial Cells. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(7), 1549-1561.

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Semen Analysis and Sperm Motility Enhancement

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After sexual abstinence for 3 days, semen samples were collected through masturbation and were liquefied for 30 min and delivered to laboratory in <60 min after ejaculation according to World Health Organization (WHO) guidelines (Menkveld, 2010). Asthenozoospermia was defined by the following criteria: progressive motility <32%; sperm concentration >15 million/ml; total motility (progressive motility + nonprogressive motility) <40%; and normal forms of sperm morphology >4%. 10% (w/v) bovine serum albumin (Sigma) was added to modified human tubular fluid (irvinesci.lnc.) medium to wash semen samples. Subsequently, the semen samples were applied to density gradient centrifugation with 90% (v/v) and 50% (v/v) Percol (GE Healthcare) at 300 g for 20 min. Spermatozoa were incubated with curcumin or dimethyl sulfoxide for 30 min at 37°C. The spermatozoa motility was measured through a computer‐assisted semen analyser (Hamilton Thorne, lnc.).

Zhou Q., Wu X., Liu Y., Wang X., Ling X., Ge H, & Zhang J. (2019). Curcumin improves asthenozoospermia by inhibiting reactive oxygen species reproduction through nuclear factor erythroid 2‐related factor 2 activation. Andrologia, 52(2), e13491.

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