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Prostate specific antigen

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The Prostate Specific Antigen (PSA) is a laboratory equipment used for the measurement of PSA levels in blood samples. PSA is a protein produced by the prostate gland and is often used as a biomarker for the detection and monitoring of prostate-related conditions.

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Lab products found in correlation

Caspase 3, by Cell Signaling Technology (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Hybond ecl membrane, by GE Healthcare (1 mentions) P27kip, by Santa Cruz Biotechnology (1 mentions) Lc3 2, by Abcam (1 mentions) P21waf1, by Cell Signaling Technology (1 mentions) Cyclin b1, by Santa Cruz Biotechnology (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Cyclin d1, by Santa Cruz Biotechnology (1 mentions) C src, by Cell Signaling Technology (1 mentions) Ar441, by Santa Cruz Biotechnology (1 mentions) Smad3, by Thermo Fisher Scientific (1 mentions) Enzalutamide, by Selleck Chemicals (1 mentions) Phospho ampkα, by Cell Signaling Technology (1 mentions) And β actin, by Merck Group (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) Caspase 7, by Cell Signaling Technology (1 mentions) Cleaved caspase 7, by Cell Signaling Technology (1 mentions) Extracellular signal regulated kinase erk 1 2, by Cell Signaling Technology (1 mentions)

3 protocols using prostate specific antigen

1

Protein Expression Analysis by Western Blot

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Cells were lysed in SDS buffer, and 10 μl of protein was resolved in 12% polyacrylamide gels and transferred onto Hybond ECL membranes (GE Healthcare, Piscataway NJ). ELK4 (Abcam, Cambridge, UK), KLK2 (Santa Cruz Biotechnology, Santa Cruz, CA), cyclin B1 (Santa Cruz), cyclin D1 (Santa Cruz), CDK2 (Santa Cruz), CDK4 (Santa Cruz), caspase 3 (Cell Signaling), cleaved caspase 3 (Cell Signaling), prostate specific antigen (PSA) (Dako, Glostrup, Denmark), AR (Santa Cruz), p21WAF1(Cell Signaling), p27KIP (Santa Cruz), and LC3-II (Abcam) antibodies were used to assess their protein expression levels. Beta actin expression was evaluated to confirm equal amount of protein loading using a monoclonal anti-beta-actin antibody (Sigma-Aldrich).
Etani T., Suzuki T., Naiki T., Naiki-Ito A., Ando R., Iida K., Kawai N., Tozawa K., Miyata N., Kohri K, & Takahashi S. (2014). NCL1, a highly selective lysine-specific demethylase 1 inhibitor, suppresses prostate cancer without adverse effect. Oncotarget, 6(5), 2865-2878.
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2

Integrin and Signaling Pathway Analysis

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Synthetic androgen R1881 was from Perkin-Elmer. Enzalutamide was from Selleck. The following rabbit Abs (pAbs) were used: AR (N20), phospho-ERK1 (Santa Cruz); Smad3 (Invitrogen); p-src (Y416), Akt, p-Akt, p-JNK and p-p38 (Cell Signaling); prostate specific antigen (PSA) (DAKO Cytomation). A goat Ab against p38 was from Santa Cruz. A rabbit monoclonal Ab against β6 integrin, B1, was from Dr. Dean Sheppard. The following mouse monoclonal Abs (mAbs): AR (441) (Santa Cruz); β1 integrin (C-18) and JNK (BD Biosciences); c-src (Cell Signaling); β1 integrin (TS2/16) and β3 integrin (AP3) are from ATCC; β5 integrin (P1F6) from Life Technologies, Inc.; αv integrin (VNR147) and α5 integrin (P1D6); CK8 (Boehringer Mannheim), CK18 (Sigma); β6 integrin Abs 6.2A1, ch2A1 and 6.3G9 were previously described (21 (link)); β6 integrin Ab 10D5 from Chemicon. Purified non-immune mouse IgGs (mIgG) from Pierce, 1E6 (IgG1) from Biogen and 1C10 were used as negative controls.
Lu H., Wang T., Li J., Fedele C., Liu Q., Zhang J., Jiang Z., Jain D., Iozzo R.V., Violette S.M., Weinreb P.H., Davis R.J., Gioeli D., FitzGerald T.J., Altieri D.C, & Languino L.R. (2016). αvβ6 Integrin Promotes Castrate-Resistant Prostate Cancer Through JNK1-Mediated Activation of Androgen Receptor. Cancer research, 76(17), 5163-5174.
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3

Protein Expression Analysis of Frozen Tissues

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Proteins from frozen tissues or harvested cells were extracted as previously outlined [20 (link)]. The antibodies used were against the following antigens: fatty acid synthase (FAS), adenosine monophosphate-activated protein kinase (AMPK)α, phospho-AMPKα, cyclin D1, p21, cleaved caspase 3, caspase 3, cleaved caspase 7, caspase 7, p38 mitogen-activated protein kinase (MAPK), phospho-p38 MAPK, extracellular signal-regulated kinase (ERK)1/2, and phospho-ERK1/2 (Cell Signaling Technology, MA, USA); cdk4 (Thermo Fisher Scientific, MA, USA); prostate-specific antigen (PSA; DAKO, Tokyo, Japan); and AR, NKX3.1, and β-actin (Sigma-Aldrich, St Louis, MO, USA). Each band intensity was semi-quantified using ImageJ 1.51K (National Institute of Health, Bethesda, MD, USA).
Yeewa R., Naiki-Ito A., Naiki T., Kato H., Suzuki S., Chewonarin T, & Takahashi S. (2020). Hexane Insoluble Fraction from Purple Rice Extract Retards Carcinogenesis and Castration-Resistant Cancer Growth of Prostate through Suppression of Androgen Receptor Mediated Cell Proliferation and Metabolism. Nutrients, 12(2), 558.
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