The analysis was performed on a Model BX61 Fluorescence Microscope (Olympus Corp., Tokyo, Japan), Spinning Disk Olympus (DSU) system, coupled to an ORCA-R2 camera (Hamamatsu Photonics KK, Japan) and CellSens Dimension software v1.9 (Olympus Corp., Tokyo, Japan). Fluorescence emission was obtained by excitation with a xenon lamp. Emission was collected with a DAPI filter (450 to 500 nm).
Cellsens dimension software v1
Manufactured by Olympus
Sourced in Japan
CellSens Dimension software v1.9 is a microscope imaging and analysis software developed by Olympus. Its core function is to provide users with tools for image capture, processing, and analysis of microscopic samples.
Automatically generated - may contain errors
2 protocols using cellsens dimension software v1
1
Fluorescence Microscopy of DAPI-Stained Samples
2
Bioluminescence Imaging of Pupation
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Homozygotes of engrailed-Gal4 and UASp-Pmat strains were crossed, and collect progenies (third insular lave) and feed yeast paste containing 3 mM D-luciferin for more than 6 h. Prepupa (white pupa) was placed on 35-mm glass-bottom dish, and bioluminescence images were captured by LV200 attached with ImagEM EM-CCD camera (EM gain 1200, exposure time 30 s), UPLFLN10×PH objective lens (NA 0.30, Olympus), motorized focus drive, HP1762 (Prior, Cambridge, UK), and Z-focus controller, ES10ZE (Prior). This system was controlled by CellSens Dimension software v. 1.9 (Olympus).
Fifty-one images were captured at steps of every 10 μm for 500-μm thickness, and all-focused images (transmitted light option) were generated by CellSens Dimension. For time-lapse imaging, the capture sequence was repeated at 30-min intervals for 93 h.
Fifty-one images were captured at steps of every 10 μm for 500-μm thickness, and all-focused images (transmitted light option) were generated by CellSens Dimension. For time-lapse imaging, the capture sequence was repeated at 30-min intervals for 93 h.
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