Ir700dx nhs ester

Manufactured by LI COR

The IR700DX-NHS ester is a near-infrared fluorescent dye that can be used for labeling proteins and other biomolecules. It contains an N-hydroxysuccinimide (NHS) ester group, which allows for covalent attachment to primary amines on the target molecule.

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Lab products found in correlation

Celltiter glo luminescent cell viability assay kit, by Promega (4 mentions) Synergy h4 hybrid multi mode microplate reader, by Agilent Technologies (4 mentions) 96 well optical black plates, by Thermo Fisher Scientific (4 mentions) Ivis lumina xr in vivo imaging system, by PerkinElmer (3 mentions) Axio observer fluorescent microscopy system, by Zeiss (2 mentions) Jwh 133, by Cayman Chemical (1 mentions) Mda mb 231 human breast cancer cells, by American Type Culture Collection (1 mentions) Ir800cw maleimide, by LI COR (1 mentions) Cary 100 bio uv vis spectrophotometer, by Agilent Technologies (1 mentions) Apoptag in situ apoptosis detection kit, by Merck Group (1 mentions) Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (1 mentions) Lct premier mass spectrometer, by Waters Corporation (1 mentions) Avance 3 400 mhz system, by Bruker (1 mentions) Avance 3 400 mhz, by Brucker (1 mentions)

5 protocols using ir700dx nhs ester

Photodynamic Therapy for Cancer

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The solvents used are of ACS or HPLC grade. The photosensitizer, IR700DX-NHS ester, was purchased from LI-COR Bioscience (Lincoln, NE). JWH-133 was purchased from Cayman chemical (Ann Arbor, MI). The following instruments, supplies and assay kits were used for in vitro and in vivo studies: Synergy™ H4 Hybrid Multi-Mode Microplate Reader (BioTek, Winooski, VT), Zeiss Axio Observer fluorescence microscopy system (Zeiss, Jena, Germany), 96-well optical black plates (Fisher Scientific, Pittsburgh, PA), CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, WI), Agilent Technology 6130 LCMS (Santa Clara, CA) and IVIS Lumina XR in vivo imaging system (PerkinElmer, Waltham, MA). MDA-MB-231 human breast cancer cells were purchased from ATCC (Catalog # HTB-26).

Zhang J., Zhang S., Liu Y., Su M., Ling X., Liu F., Ge Y, & Bai M. (2018). Combined CB2 Receptor Agonist and Photodynamic Therapy Synergistically Inhibit Tumor Growth in Triple Negative Breast Cancer. Photodiagnosis and photodynamic therapy, 24, 185-191.

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Affibody Bioconjugation for Imaging

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The EGFR‐targeted affibody (Z_EGFR:03115‐Cys) and the non‐specific affibody (Z_Taq) were provided through our ongoing collaboration with AffibodyAB (Stockholm, Sweden). The hydrophilic IR700DX–maleimide used for affibody conjugation, IR700DX–NHS ester, IR700DX–carboxylate and IR800CW–maleimide were purchased from LI‐COR Biosciences (Lincoln, NE).

Burley T.A., Mączyńska J., Shah A., Szopa W., Harrington K.J., Boult J.K., Mrozek‐Wilczkiewicz A., Vinci M., Bamber J.C., Kaspera W, & Kramer‐Marek G. (2018). Near‐infrared photoimmunotherapy targeting EGFR—Shedding new light on glioblastoma treatment. International Journal of Cancer, 142(11), 2363-2374.

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Breast Cancer Cell Analysis Protocol

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The solvents used are of ACS grade or HPLC grade. The PS, IR700DX-NHS ester, was purchased from LICOR Bioscience (Lincoln, NE). ¹H NMR spectra were recorded on a Bruker Avance III 400 MHz system. Mass spectra were recorded on a Waters LCT Premier mass spectrometer. UV/Vis absorption spectra were recorded on a Cary 100 Bio UV–vis Spectrophotometer (Agilent Technologies, Santa Clara, CA). Fluorescence emission spectra were recorded on a Cary Eclipse fluorescence spectrophotometer (Agilent Technologies, Santa Clara, CA). MDA-MB-231 and MCF-7 human breast cancer cells were gifts from Dr. Carolyn J. Anderson’s lab (University of Pittsburgh, Pittsburgh, PA). The following instruments, supplies and assay kits were used: Synergy^™ H4 Hybrid Multi-Mode Microplate Reader (BioTek, Winooski, VT), Zeiss Axio Observer fluorescent microscopy system (Zeiss, Jena, Germany), 96-well optical black plates (Fisher Scientific, Pittsburgh, PA), CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, WI), ApopTag^® In Situ Apoptosis Detection Kits (EMD Millipore, Billerica, MA), and IVIS Lumina XR in vivo imaging system (PerkinElmer, Waltham, MA).

Zhang S., Yang L., Ling X., Shao P., Wang X., Edwards W.B, & Bai M. (2015). Tumor Mitochondria-targeted Photodynamic Therapy with a Translocator Protein (TSPO)-specific Photosensitizer. Acta biomaterialia, 28, 160-170.

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Photosensitizer-based Cell Viability Assays

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The solvents used are of ACS or HPLC grade. The photosensitizer, IR700DX-NHS ester, was purchased from LI-COR Bioscience (Lincoln, NE). The following instruments, supplies and assay kits were used for in vitro and in vivo studies: Synergy^™ H4 Hybrid Multi-Mode Microplate Reader (BioTek, Winooski, VT), Zeiss Axio Observer fluorescent microscopy system (Zeiss, Jena, Germany), 96-well optical black plates (Fisher Scientific, Pittsburgh, PA), CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, WI), apoptosis/necrosis cell detection kit (PromoKine, Heidelberg, Germany), IVIS Lumina XR in vivo imaging system (PerkinElmer, Waltham, MA).

Yang L., Zhang S., Ling X., Shao P., Jia N, & Bai M. (2017). Multilayer Photodynamic Therapy for Highly Effective and Safe Cancer Treatment. Acta biomaterialia, 54, 271-280.

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Synthesis and Characterization of Fluorescent Probe

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All solvents used are of ACS or HPLC grade (Fisher Scientific, Pittsburgh, Pennsylvania). The PS, IR700DX-NHS ester, was purchased from LI-COR Bioscience (Lincoln, Nebraska). 1 H and 13 C NMR spectra were recorded at 25°C on the Brucker Avance III 400 MHz. Unless otherwise specified, chemical shifts δ were expressed in parts per million (ppm) based on tetramethylsilane (TMS) in CDCl 3 (δ 0.00 ppm), residue dimethyl sulfoxide (DMSO) (δ 2.50 ppm), or residue CHCl 3 (δ 77.16 ppm), and coupling constants J are given in Hz. Coupling patterns are abbreviated as s (singlet), d (doublet), t (triplet), q (quartet), quin (quintet), dd (doublet of doublets), ddd (doublet of doublet of doublets) and m (multiplet). Electrospray mass spectra were recorded on SYNAPT G2S TOF-MS mass spectrometer (Waters, Milford, Massachusetts) in positive ion mode. 7-Diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM) was obtained from Fischer Scientific. The following instruments, supplies and assay kits were used for in vitro studies: Synergy™ H4 Hybrid Multi-Mode Microplate Reader (BioTek, Winooski, VT), 96-well optical black plates (Fisher Scientific), CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, Wisconsin).

Ling X., Zhang S., Liu Y, & Bai M. (2018). Light-activatable cannabinoid prodrug for combined and target-specific photodynamic and cannabinoid therapy. Journal of biomedical optics, 23(10).

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