β glucuronidase type 9 a from e coli

Manufactured by Merck Group

Sourced in United States

β-glucuronidase (Type IX-A from E. coli) is an enzyme that catalyzes the hydrolysis of β-D-glucuronides. It is isolated from Escherichia coli.

Automatically generated - may contain errors

Lab products found in correlation

Dom 1, by Merck Group (3 mentions) Dontest wbtm immunoaffinity columns, by VICAM (2 mentions) Waters 2795 hpc separation module, by Waters Corporation (2 mentions) Ultrapure water, by Merck Group (1 mentions) Hplc grade methanol, by Carlo Erba (1 mentions) Ethyl acetate, by ITW Reagents (1 mentions) Formic acid, by ITW Reagents (1 mentions) Milli q integral system, by Merck Group (1 mentions) Potassium hydroxide koh, by Merck Group (1 mentions) Don standard, by Merck Group (1 mentions)

Close spelling variants of this product

β-glucuronidase from E. coli β-glucuronidase type IX A (β-glucuronidase), E coli β-glucuronidase Type IX-A β-glucuronidase Type IX Type A

4 protocols using β glucuronidase type 9 a from e coli

Quantitative Analysis of Deoxynivalenol in Urine

Check if the same lab product or an alternative is used in the 5 most similar protocols

Methanol HPLC grade (Carlo Erba Reagenti, Cornaredo, MI, Italy) and ultrapure water (Millipore, Burlington, MA, USA) were used for the LC-MS analysis.
In order to perform the planned validation study and the analytical work on collected urine samples, the standard of DON (product number: D0156, 1mg), U-[¹³C₁₅]-DON (99.0% ¹³C) and DOM-1 (product number: 34135, 2 mL) were purchased from Sigma (Saint Louis, MI, USA). The enzyme β-glucuronidase (Type IX-A from E. coli; product number: G7396—2MU) was purchased from Sigma too. The IAC DONtest WBTM (product number: G1066) were purchased from Vicam (Milford, MA, USA).

De Santis B., Debegnach F., Miano B., Moretti G., Sonego E., Chiaretti A., Buonsenso D, & Brera C. (2019). Determination of Deoxynivalenol Biomarkers in Italian Urine Samples. Toxins, 11(8), 441.

+ Open protocol

+ Expand

Quantification of Lipid Mediators

Check if the same lab product or an alternative is used in the 5 most similar protocols

AAPH (2,2′-Azobis [2-methylpropionamidine] dihydrochloride), adrenic acid (AdA, 22:4 n-6), β-glucuronidase type IX-A from E. coli, and potassium hydroxide (KOH) were purchased from Sigma Aldrich Química S.A. (Madrid, Spain). Methanol (MeOH), ethanol (EtOH), n-heptane (C₇H₁₆), and acetonitrile (CH₃CN) at LC-MS grade were purchased from J.T. Baker (Phillipsburg, NJ, USA). Formic acid (98% w/w) and ethyl acetate (analytical grade) were from Panreac (Barcelona, Spain). Dulbecco’s phosphate buffered saline (PBS) containing 137 mM of NaCl, 2.7 mM of KCl, 10 mM of sodium hydrogen phosphate, and 1.8 mM of potassium dihydrogenphosphate at pH 7.4 was prepared dissolving the pre-weighted tablet from VWR (Radnor, PA, USA) in 100 mL of water. Water was ultrapure H₂O generated on a Milli-Q® integral system from Merck Millipore (Darmstadt, Germany). Deuterated prostaglandin F_2α (PGF_2α-D4) with purity ≥ 98% and deuterated incorporation ≥ 99% was purchased from Cayman Chemical Company (Ann Arbor, MI, USA) and employed as internal standard (IS).

Sánchez-Illana Á., Shah V., Piñeiro-Ramos J.D., Di Fiore J.M., Quintás G., Raffay T.M., MacFarlane P.M., Martin R.J, & Kuligowski J. (2019). Adrenic acid non-enzymatic peroxidation products in biofluids of moderate preterm infants. Free radical biology & medicine, 142, 107-112.

+ Open protocol

+ Expand

Analysis of DON and Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following chemicals were used for the analysis of DON and its metabolites: ¹³C labelled DON standard (Sigma, Saint Louis, MO, USA; product number: 34128, 1.2 mL); DON (Sigma, Saint Louis, MO, USA; product number: D0156, 1 mg); β-glucuronidase (Type IX-A from E. coli; Sigma, Saint Louis, MO, USA; product number: G7396-2MU); DOM-1 (Sigma, Saint Louis, MO, USA; product number: 34135, 2 mL); and DONtest WB^TM immunoaffinity columns (Vicam, Milford, MA, USA; product number: G1066). The equipment used was a Waters 2795 HPC Separation Module (Waters Corp., Milford, MA, USA) with a Quattro Micro Triple Quadrupole Mass Spectrometer (Micromass UK Ltd., Manchester, UK).

Papageorgiou M., Wells L., Williams C., White K., De Santis B., Liu Y., Debegnach F., Miano B., Moretti G., Greetham S., Brera C., Atkin S.L., Hardie L.J, & Sathyapalan T. (2018). Assessment of Urinary Deoxynivalenol Biomarkers in UK Children and Adolescents. Toxins, 10(2), 50.

+ Open protocol

+ Expand

Quantitative Analysis of DON and Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

DON and its metabolites were analysed using 13 C labelled DON standard (Sigma, Saint Louis, MI, USA; product number: 34128, 1.2 mL), DON (Sigma, Saint Louis, MI, USA; product number: D0156, 1 mg), β-glucuronidase (Type IX-A from E. coli; Sigma, Saint Louis, MI, USA; product number: G7396 -2MU), DOM-1 (Sigma, Saint Louis, MI, USA; product number: 34135, 2 mL), and DON test WB TM immunoaffinity columns (Vicam, Milford, MA, USA; product number: G1066). All analyses were conducted on a Waters 2795 HPC Separation Module (Waters Corp., Milford, MA, USA) with a Quattro Micro Triple Quadrupole Mass Spectrometer (Micromass UK Ltd., Manchester, UK).

Papageorgiou M., Wells L., Williams C., White K.L.M., De Santis B., Liu Y., Debegnach F., Miano B., Moretti G., Greetham S., Brera C., Atkin S.L., Hardie L.J, & Sathyapalan T. (2018). Occurrence of deoxynivalenol in an elderly cohort in the UK: a biomonitoring approach. Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 35(10).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!