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Ikzf3

Manufactured by Merck Group
Sourced in United States

IKZF3 is a laboratory equipment product. It is a protein that functions as a transcriptional regulator and plays a role in the development and differentiation of various cell types. The core function of IKZF3 is to modulate gene expression, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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2 protocols using ikzf3

1

Comprehensive Molecular Profiling Protocol

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Lenalidomide, pomalidomide, palbociclib, melphalan, bortezomib and dexamethasone were obtained from SelleckChem. BSJ-03-123, YKL-06-102 and CST528 were synthesized according to Brand et al. 2019 and Steinebach et al. 2020.
Primary antibodies used for Western blotting from Cell Signaling (Danvers, USA) include CDK6 (clone DCS83, #3136, RRID:AB_2229289, 1:2000), CDK4 (clone D9G3E, #12790, RRID:AB_2631166, 1:1000), Rb (clone 4H1, #9309, RRID:AB_823629, 1:1000), Phospho-Rb (Ser807/811) (#9308, RRID:AB_331472, 1:1000), IKZF3 (clone D1C1E, #15103, RRID:AB_2744524, 1:1000), IKZF1 (clone D6N9Y, #14859, RRID:AB_2744523, 1:1000), IRF4 (clone D43H10, #4299, RRID:AB_10547141, 1:1000), c-Myc (clone D84C12, #5605, RRID:AB_1903938, 1:1000), RRM1 (clone D12F12, #8637, RRID:AB_11217623, 1:1000), RRM2 (clone E7Y9J, #65939, 1:1000), anti-rabbit IgG HRP-linked antibody (#7074, 1:5000), anti-mouse IgG HRP-linked antibody (#7076, 1:5000); antibodies from Sigma-Aldrich (St. Louis, USA) include anti-α-Tubulin (#T5168, RRID:AB_477579, 1:7000); antibodies from Santa Cruz Biotechnology (Dallas, USA) include CDK6 (clone B-10, sc-7961, 1:1000), TRIP13 (clone A-7, sc-514285, 1:1000); antibodies from Abcam (Cambridge, UK) include anti-β-actin (ab20272, 1:10,000).
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2

Baicalein Compound Preparation and CRBN Antibodies

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Baicalein (purity, 98%) was purchased from Sigma-Aldrich (USA) and dissolved in dimethylsulphoxide (DMSO) (Shanghai Sidande Biotechnology Co. Shanghai, China). The final concentration of DMSO in all experiments was <0.1%. Monoclonal anti-CRBN, anti-IKZF1, and IKZF3 were purchased from Sigma-Aldrich (USA).
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