Cellic ctec2 preparation

Cellic^® CTec2 preparation (Novozymes, Franklinton, NC, USA) containing a complex of cellulolytic enzymes (75 FPU/mL activity) was used in the enzymatic hydrolysis of cellulose. The preparation was applied according to the manufacturer’s instructions at pH 5.5 and 50 °C. Selected fermentation media were also prepared using Viscozyme^® L (Novozymes, Bagsvaerd, Denmark). This preparation exhibits endo-β-glucanase (xylanase, cellulase, hemicellulase) activity, 100 FBG g-1, and hydrolyses β-(1,3)- or β-(1,4)-glycosidic linkages in β-D-glucans. It was used according to the manufacturer’s instructions at a dose of 10 FGB/g DW at pH 5.5 and 50 °C.

Cellic® CTec2 preparation (Novozymes, Franklinton, NC, USA) containing a complex of cellulolytic enzymes with 75 FPU (filter-paper units)/mL activity was used in the enzymatic hydrolysis of cellulose. The preparation was applied according to the manufacturer’s instructions at pH 5.5 and 50 °C. In the preparation of fermentation media, Viscozyme® L (Novozymes, Bagsvaerd, Denmark) was additionally used. This preparation exhibits endo-β-glucanase (xylanase, cellulase, hemicellulase) activity, 100 FBG/g, and hydrolyses β-(1,3)- or β-(1,4)-glycosidic linkages in β-D-glucans. It was used according to the manufacturer’s instructions at a dose of 8 FGB (beta glucanase units)/g DW, at pH 5.5 and 50 °C.