Anti integrin β1

Commercially available antibodies were used: anti-DDX4/Cvh (ab13840, Abcam, Cambridge, UK); Goat Anti-Mouse IgG (Cy3 labeled, Bio-Synthesis, Inc., Louisville, KY, USA; dilution ratio:1:100); anti-NANOG and anti-OCT4 (Abcam, dilution ratio 1:100); anti-SSEA (BioLegend, San Diego, CA, USA, dilution ratio 1:100); anti-integrin α6 and anti-integrin β1 (BioLegend, dilution ratio 1:100).
Dulbecco’s modified Eagle medium (DMEM, 41965062) and fetal bovine serum (FBS, 10100-147) from Gibco (Grand Island, NY, USA); FuGENE^® HD (E2311) and Dual-Luciferase^® Reporter Assay System from Promega (Madison, WI, USA); Leukemia Inhibit Factor from mouse (mLIF, L5158), Fibroblast Growth Factor-Basic human (bFGF, F0291), Stem Cell Human (SCF, S7901); TSA from Sigma (St. Louis, MO, USA); PARIS™ from Ambion (Austin, TX, USA); retinoic acid (R2625) from Sigma.

The following antibodies were used at the dilutions specified—immunofluorescent (IF) or Western blot analysis (WB): anti-Arl13b, 1:2000 IF (clone N295B/66, UC Davis/NIH NeuroMab Facility), anti-Ac3 (gift from Young-Goo Han laboratory), anti-Siah2, 1:200 IF (Santa Cruz Biotechnology, cat. no. SC81787); anti-Tag1, 1:50 IF (UC Davis/NIH NeuroMab Facility, cat. no. 4D7/TAG1); anti-β-actin, 1:20000 WB (Sigma, cat. no. A2228); and anti-phospho-Mek1/2, 1:500 IF (Cell Signaling, cat. no. 9121); anti-H-Ras, 1:500 WB (Abcam, cat. no. ab32417); anti-N-Ras, 1:500 WB (Abcam, cat. no. ab77392); anti-K-Ras, 1:2000 WB (Proteintech, cat. no. 12063-1-AP); anti-vitronectin, 1:200 IF (Thermofisher cat. no. MA5-24083); anti-integrin β1 (Biolegend. Clone HMβ1-1); anti-myc 1:2000 WB (Thermofisher, cat. no. PA1-981); anti-HA 1:2000 WB (Biolegend, clone 16B12).