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3 protocols using tetrabutylammoniumfluoride hydrate

1

Rare-earth metal complexes synthesis

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Yttrium(III) acetate hydrate 99.9%, yttrium(III)
trifluoroacetate 99%, yttrium(III) nitrate hexahydrate 99.8%, yttrium(III)
chloride hexahydrate 99.99%, samarium(III) acetate hydrate 99.9%,
europium(III) acetate hydrate 99.9%, lutetium(III) acetate hydrate
99.9%, citric acid 99%, maleic acid ≥99%, citraconic acid 98%,
tetramethylammonium hydroxide 25% v/v in water, tetrabutylammonium
fluoride hydrate 98%, and ammonium fluoride >99.99% were purchased
from Sigma-Aldrich. Ethanol 96% from Panreac, acetone 99.5% from Scharlau,
and deuterium oxide 99.90% D were purchased from euriso-top. All reagents
were used as received without further purification.
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2

Stimuli-Responsive Polymeric Formulations

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Sodium alginate was obtained at Wako Pure Chemical Industries (11 kDa, Osaka, Japan). Folic acid, tetrabutylammonium hydroxide solution (TBAOH), tetrabutylammonium fluoride hydrate (TBAF), 1,1′-carbonyldiimidazole (CDI), soybean oil, 5-aminolevulinic acid hydrochloride (5ALA, 98%), sorbitan oleate (Span 80), and Polysorbate 80 (Tween 80) were supplied from Sigma-Aldrich (St Louis, MO, USA). Hydrogen peroxide (H2O2, 30.0–35.5%), dimethyl sulfoxide (DMSO, 99.8%), sodium carbonate (Na2CO3), and hydrochloric acid (HCl, 35.0–37.0%) were purchased from Samchun (Gyeonggi-do, Korea). 2,4,6-trinitrobenzene sulfonic acid (TNBSA, 5% w/v) solution, and bicinchoninic acid (BCA) assay kit were obtained from Thermo Fisher (Waltham, MA, USA). The MCF-7 (breast adenocarcinoma), A549 (lung carcinoma), and SKOV-3 (ovarian adenocarcinoma) cell lines were procured from Korea Cell Line Bank (Seoul, Korea). The human fibroblast (HFB) cell line was offered by Seoul National University Hospital (Bundang, Gyeonggi-do, Korea). RIPA lysis buffer was purchased from Biosesang (Gyeonggi-do, Korea) and Cell Counting Kit-8 (CCK-8) was acquired by Dojindo Molecular Technologies, Inc. (Kumamoto, Japan).
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3

Sodium Hyaluronate Synthesis and Characterization

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All reagents used were of analytical grade. Sodium hyaluronate (M W = 7.46 KDa) was purchased from Lifecore Biomedical (USA). AG 50W -X8 resin was purchased from Bio-Rad (USA). Dimethyl sulfoxide (DMSO), Tetrabutylammonium fluoride hydrate (TBA-F), 11-Amino-1-undecanethiol hydrochloride (AT), N-hydroxysulfosuccinimide (NHS), 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), hydrogen nicotinamide adenine dinucleotide (NADH), pyruvate, haemoglobin from bovine blood and Drabkin's reagent were acquired from Sigma-Aldrich (Italy). Fluorescein-5-thiosemicarbazide and Cy5.5 hydrazide were purchased from Life Technologies Ltd (UK). Cell Culture reagents and culture medium were purchased from Biochrom (Germany). The water used was distilled and ultrapurified (Milli-Q system).
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