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Psiren retroq expression vector

Manufactured by Takara Bio
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The PSIREN-RetroQ expression vector is a tool used for gene expression analysis. It is designed to facilitate the production of recombinant proteins in mammalian cell lines. The vector contains essential elements required for efficient gene expression and viral packaging.

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Lab products found in correlation

Psuper retro vector, by Oligoengine (1 mentions)

Close spelling variants of this product

PSIREN-retroQ-based shRNA expression vector PSIREN-RetroQ retroviral shRNA expression vector PSIREN-RetroQ retroviral short hairpin RNA (shRNA) expression vector PSIREN-RetroQ vector PSIREN-RetroQ

2 protocols using psiren retroq expression vector

1

Knockdown of SIRT7 Using Retroviral shRNA

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For KD of SIRT7, oligonucleotides encoding SIRT7 shRNA (target sequence: shSirt7, 5′-GGGACACCATTGTGCACTT-3′) were cloned into the pSIREN-RetroQ expression vector (Clontech, Mountain View, CA). After the pSIREN-RetroQ-SIRT7 or pSIREN-RetroQ-control vector was transfected into Plat-E cells, the retrovirus-containing medium was mixed with Polybrene and added to NRK-52E cells for infection. Selection was achieved using puromycin treatment (5 µg/mL).
Miyasato Y., Yoshizawa T., Sato Y., Nakagawa T., Miyasato Y., Kakizoe Y., Kuwabara T., Adachi M., Ianni A., Braun T., Komohara Y., Mukoyama M, & Yamagata K. (2018). Sirtuin 7 Deficiency Ameliorates Cisplatin-induced Acute Kidney Injury Through Regulation of the Inflammatory Response. Scientific Reports, 8, 5927.
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2

Knockdown of HIF-1α and CHOP in MIN6 cells

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For knockdown of HIF-1α, oligonucleotide encoding shRNA (target sequence: 5′-AGATGAGTTCTGAACGTCG-3′) was cloned into pSUPER.retro vector (Oligoengine). Oligonucleotide encoding CHOP shRNA (target sequence: 5′-GATTCCAGTCAGAGTTCTATG-3′) was cloned into a pSIREN-RetroQ expression vector (Clontech). After pSUPER.retro-HIF-1α, pSUPER.retro-control, pSIREN-RetroQ-CHOP, or pSIREN-RetroQ-control vector was transfected into Plat-E cells, which are a retroviral packaging cell line, retrovirus-containing medium was mixed with polybrene and added to MIN6 cells for infection. Selection was achieved by using puromycin treatment (5 µg/ml) [16] (link), [17] .
Sato Y., Inoue M., Yoshizawa T, & Yamagata K. (2014). Moderate Hypoxia Induces β-Cell Dysfunction with HIF-1–Independent Gene Expression Changes. PLoS ONE, 9(12), e114868.
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