Camkk

Primary and secondary antibodies against AMPK, phosphor-AMPK, phosphor-HSL, FAS, CaMKK, phosphor-CaMKK, LKB1, phosphor-LKB1, protein kinase A (PKA), phosphor-PKA, Sirtuin 1 (Sirt1), PPARγ, C/EBPα, C/EBPβ, anti-rabbit IgG and anti-mouse IgG were purchased from Cell Signalling Technology (Commonwealth of Massachusetts, USA). Phosphor-C/EBPβ was purchased from Abcum (Cambridge, UK). HSL and anti-mouse β-actin were purchased from Sigma (State of Missouri, USA).

Apelin 13 peptide was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Hyclone (Logan, UT, USA). Fetal bovine serum (FBS) was obtained from Sijiqing Biotechnology (Hangzhou, China). Annexin V-FITC apoptosis detection kit was obtained from Kaiji Biotechnology (Hangzhou, China). 8-Oxo-dG, MDA, SOD, GSH, CAT, IL-1β, TNF-α, COX-2, and IL-6 detection kits were purchased from Jiancheng Biotechnology (Hangzhou, China). 2,3,5-triphenyltetrazolium chloride (TTC) and dihydroethidium (DHE) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Antibodies for P-AMPK, AMPK, GSK-3β, Nrf2, P-GSK-3β, P-ACC, ACC, NQO-1, HO-1, BCL-2, Bax, cleaved caspase 3, cleaved caspase 9, IP3, PLC, CaMKK, and β-actin were purchased from Cell Signaling Technology (Beverly, MA, USA). Annexin V-FITC apoptosis detection kit was purchased from Jiancheng Biotechnology (Hangzhou, China). All other chemicals used in this experiment were the purest grade commercially available.