Btf 608a

Manufactured by Fortis Life Sciences

The BTF-608A is a laboratory instrument used for cell culture and bioreactor applications. It features precise temperature and pH control to support optimal growth conditions for cell cultures. The device includes key functionalities for maintaining a stable environment for cell-based experiments and processes.

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Lab products found in correlation

Jbw301, by Merck Group (1 mentions) Mops buffer, by Thermo Fisher Scientific (1 mentions) Brca2 h 300, by Santa Cruz Biotechnology (1 mentions) Atr n 19, by Santa Cruz Biotechnology (1 mentions) Cyclin b1 gns1, by Santa Cruz Biotechnology (1 mentions) Atm 2c1, by Santa Cruz Biotechnology (1 mentions) Patr s428, by Cell Signaling Technology (1 mentions)

2 protocols using btf 608a

BRCA1 and BCLAF1 Coimmunoprecipitation

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Coimmunoprecipitations were carried out as previously described using (BRCA1; Ab1, Millipore) or BCLAF1 (BTF-608A, Bethyl Laboratories) antibodies.

Savage K.I., Gorski J.J., Barros E.M., Irwin G.W., Manti L., Powell A.J., Pellagatti A., Lukashchuk N., McCance D.J., McCluggage W.G., Schettino G., Salto-Tellez M., Boultwood J., Richard D.J., McDade S.S, & Harkin D.P. (2014). Identification of a BRCA1-mRNA Splicing Complex Required for Efficient DNA Repair and Maintenance of Genomic Stability. Molecular Cell, 54(3), 445-459.

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Whole Cell Extract Immunoblotting

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Whole cell extracts were prepared by lysing cells in two volumes of Lysis Buffer (250 mM NaCl, 5 mM ethylenediaminetetraacetic acid (EDTA), 50 mM HEPES, 0.1% v/v NP40). A total of 60 μg of whole cell extract (WCE) was resolved on 4–12% NOVEX gels using MOPS buffer (Invitrogen). Protein was transferred to PVDF membrane (Invitrogen) and blotted for BCLAF1 (BTF 608A, Bethyl Labs); THRAP3 (A300–956A, Bethyl Labs), GAPDH (ab9485, Abcam), pATM (S1981) (ab79891 Abcam), ATM (2C1, Santa Cruz), pATR (S428) (2853, Cell Signaling), ATR (N-19, Santa Cruz), 53BP1 (NB100–34, Novus Biologicals), γH2AX (S139) (JBW301, Millipore), cyclin B1 (GNS1) (sc-245, Santa Cruz), pP53 (S15) (9284S, Cell Signaling), pKAP1 (S824) (A300–767A, Bethyl Labs), FANCD2 (FI17) (sc200–22, Santa Cruz), BRCA2 (H-300) (sc-8326, Santa Cruz), FANCL (B-11) (sc-137067, Santa Cruz), PALB2 (H-45) (sc-382436, Santa Cruz) and Rad51 (3C10) (sc-53428, Santa Cruz).

Vohhodina J., Barros E.M., Savage A.L., Liberante F.G., Manti L., Bankhead P., Cosgrove N., Madden A.F., Harkin D.P, & Savage K.I. (2017). The RNA processing factors THRAP3 and BCLAF1 promote the DNA damage response through selective mRNA splicing and nuclear export. Nucleic Acids Research, 45(22), 12816-12833.

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