Fitc or texas red

After 3, 7 and 14 days after surgery, mice were sacrificed and gastrocnemius muscles were dissected, frozen in liquid nitrogen and sectioned at 5 μm thickness. The capillaries were visualized by immunofluorescent staining with anti‐CD31 (Abcam). Anti‐F4/80 and anti‐CD206 antibodies (Abcam) were used to identify M2 macrophages. Respective secondary antibody conjugated with FITC or Texas Red (Vector Laboratories) was used. The slides were coverslipped using VECTASHIELD Mounting Medium with DAPI (Vector Laboratories). Imaging of the fluorescence of the stained sections was performed with the confocal microscope LSM710. Frozen sections were examined histochemically (haematoxylin/eosin staining; Sigma‐Aldrich). Analyses of the specimens were conducted using Nikon Eclipse 80i microscope (Nikon Instruments Inc).

Antibodies against keratin-10 (K-10; PRB-159P; Covance), E-cadherin (H-108; Santa Cruz), involucrin (SY5; Abcam), GR (M-20 or H-300; Santa Cruz Biotechnology), p23 (JJ3; a gift from David O. Toft, Mayo Clinic), Fkbp51 (HI51B; a gift from Marc Cox, University of Texas at El Paso), loricrin, (PRB-145P; Covance), filaggrin (PRB-417P; Covance) and GAPDH (Abcam) were used for immunohistochemistry, immunofluorescence or immunoblotting. Secondary biotin-conjugated (Vector Laboratories), peroxidase-conjugated (DAKO) or FITC- or Texas Red- (Vector Laboratories) conjugated anti-rabbit or anti-mouse antibodies were used for immunohistochemistry, immunoblotting or immunofluorescence, respectively.