Antibodies used were as follows: rabbit anti-FANCD2 (NB100-182; Novus), 4 µg per IP; rabbit anti-SF3B1 (A300-996A; Bethyl), 1 µg per IP; rabbit anti-SF3B1 (Eurogentec), 25 µg per IP; rabbit anti-DHX15 (A300-389A; Bethyl), 2 µg per IP; anti-FLAG M2 Affinity Gel (A2220; Sigma-Aldrich), 20 µl per IP; anti-FLAG M2 magnetic beads (M8823; Sigma-Aldrich) 20 µl per IP; and rabbit IgG (NI01; Calbiochem), according amount to be comparable to the experiment.
Rabbit anti sf3b1
Manufactured by Eurogentec
Rabbit anti-SF3B1 is a primary antibody that recognizes the SF3B1 protein. SF3B1 is a component of the spliceosome, a complex involved in the processing of pre-mRNA. This antibody can be used in various applications to detect and study the SF3B1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti fancd2 nb100 182, by Novus Biologicals (2 mentions)
Rabbit anti dhx15, by Fortis Life Sciences (2 mentions)
Mouse anti sc35, by BD (2 mentions)
Anti flag m2 magnetic bead, by Merck Group (1 mentions)
Anti flag m2 affinity gel, by Merck Group (1 mentions)
Rabbit anti flag f7425, by Merck Group (1 mentions)
Anti mouse hrp linked, by Cell Signaling Technology (1 mentions)
Rabbit anti topbp1, by Fortis Life Sciences (1 mentions)
Rabbit anti fanca, by Fortis Life Sciences (1 mentions)
Rabbit anti u2af65 ab37530, by Abcam (1 mentions)
Mouse anti tubulin, by Merck Group (1 mentions)
Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 546 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
3 protocols using rabbit anti sf3b1
1
Immunoprecipitation Protocol for Cellular Factors
2
Antibody Characterization for DNA Damage Response
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Antibodies were as follows: rabbit anti-FANCD2 (NB100-182; Novus) at 1:10,000; rabbit anti-FANCI (A301-254A; Bethyl) at 1:1,000; rabbit anti-SF3B1 (A300-996A; Bethyl) at 1:2,000; rabbit anti-SF3B1 (Eurogentec) at 1:1,000; rabbit anti-SF3A3 (A302-507A; Bethyl) at 1:2,000; rabbit anti-FLAG (F7425; Sigma-Aldrich) at 1:1,000; rabbit anti-DHX15 (A300-389A; Bethyl) at 1:5,000, mouse anti-SC35 (556363; BD Pharmingen) at 1:500; rabbit anti-U2AF65 (ab37530; Abcam) at 1:250; rabbit anti-pSer345(Chk1) (2348L, rabbit; Cell Signaling Technology) at 1:1,000; mouse anti-Chk1 (Sc-8408; Santa Cruz Biotechnology) at 1:1,000; mouse anti-tubulin (T5168; Sigma-Aldrich) at 1:10,000; anti-rabbit HRP-linked (7074; Cell Signaling Technology) at 1:2,500; anti-mouse HRP-linked (7076; Cell Signaling Technology) at 1:2,500; rabbit anti-Nbs1 (NB100-143; Novus Biologicals) at 1:1,000; mouse anti-PCNA (P8825; Sigma-Aldrich) at 1:3,000; rabbit anti-TopBP1 (A300-111A; Bethyl) at 1:1,000; rabbit anti-RNA-PolII (N-20) (sc-899; Santa Cruz Biotechnology) at 1:1,000; rabbit anti-TFIP11 (A302-548A; Bethyl) at 1:1,000; rabbit anti-SPF45 (A302-548A; Bethyl) at 1:1,000; rabbit anti-FANCA (A301-980A; Bethyl) at 1:2,000; and rabbit anti-PRP8 (GTX108046; GeneTex) at 1:1,000.
3
Immunofluorescent Labeling of Nuclear Proteins
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Antibodies were as follows: mouse anti-SC35 (556363; BD Pharmingen) at 1:300; rabbit anti-SF3B1 (Eurogentec) at 1:200; and Alexa Fluor 546 goat anti–mouse IgG and Alexa Fluor 488 goat anti–rabbit IgG (A11030 and A11008 ; Invitrogen) at 1:500.
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