Annexin 5 pi assay kit

Manufactured by BD

Sourced in United States

The Annexin V/PI assay kit is a laboratory tool used to detect and quantify apoptosis, a process of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a DNA-binding dye, to identify cells in different stages of apoptosis.

Automatically generated - may contain errors

Lab products found in correlation

Trypsin, by Thermo Fisher Scientific (1 mentions) Cation adjusted mueller hinton broth, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Xtt cell proliferation assay kit, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Accuri c6 cytometer, by BD (1 mentions) Propidium iodide, by MultiSciences Biotech (1 mentions) Facscalibur, by BD (1 mentions)

Close spelling variants of this product

Annexin V/PI Annexin/PI assay Annexin V assay Annexin V kit Annexin V

4 protocols using annexin 5 pi assay kit

Nanoparticle Synthesis and Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Silver nitrate (Ag(NO₃)), sodium boron hydride (NaBH₄), trisodium citrate, dimethylsulfoxide (DMSO), and XTT cell proliferation assay kit were purchased from Sigma (St. Louis, USA). Dulbecco's Modified Eagle's Medium (DMEM), penicillin-streptomycin, trypsin, and fetal bovine serum (FBS) were purchased from Gibco (USA). 20x phosphate-buffered saline (PBS) was purchased from Thermo Fisher (USA), cation-adjusted Mueller-Hinton broth (CAMHB) and 2,3,5-triphenyltetrazolium chloride (TTC) were purchased from Merck (Germany), and annexin V/PI assay kit BD (USA) and paclitaxel were purchased from Sandoz (Germany).

Tunç T., Hepokur C, & Kari̇per A. (2024). Synthesis and Characterization of Paclitaxel-Loaded Silver Nanoparticles: Evaluation of Cytotoxic Effects and Antimicrobial Activity. Bioinorganic Chemistry and Applications, 2024, 9916187.

+ Open protocol

+ Expand

Annexin V-PI Assay for Cell Death Mechanism

Check if the same lab product or an alternative is used in the 5 most similar protocols

Annexin V PI assay kit (BD Pharmingen^TM, 559763) was used to identify the cell death mechanism induced by AlPcS₄Cl-PDT. The assay gives an indication of cell death either as necrotic or apoptotic. The test makes use of fluorescently labelled dyes which are then read using a flow cytometer quantifying the amount of fluorescently labelled cells. Flow cytometric analysis was performed on the BD flow cytometer (BD Accuri^TM C6 Cytometer), and Annexin V-FITC and propidium iodide (PI) were detected as a green and red FL, respectively [39 ].

Crous A, & Abrahamse H. (2021). Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells. Royal Society Open Science, 8(9), 210148.

+ Open protocol

+ Expand

Apoptosis Evaluation in A549 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The levels of apoptotic cells in the different treatment conditions were determined using double labeling for Annexin V and propidium iodide (PI) (Annexin V/PI assay kit, BD bioscience, US) and detection with flow cytometry according to the manufacturer’s protocol. The A549 cells plated at a density of 1 × 10⁵ cells/well in 6-well plates and then challenged with 0.1, 1 and 10 μg/mL PM2.5 followed by tea polyphenol treatment as described previously. At the end of the assay, the cells were harvested, washed with cold PBS, and incubated in the dark with 1× Annexin V working solution containing PI (1 μg/mL final concentration) for 5 min at room temperature. The cells were exposed to binding buffer according to the manufacturer’s recommended protocol (400 μL of 1× binding buffer and sample cells). The proportion of apoptotic cells was determined using CellQuest software on a flow cytometer with gates set at default sensitivity to determine single and double-labeled populations. The cell number was set at 2×10⁴ per test.

Zhang Y., Darland D., He Y., Yang L., Dong X, & Chang Y. (2018). REDUCTION OF PM2.5 TOXICITY ON HUMAN ALVEOLAR EPITHELIAL CELLS A549 BY TEA POLYPHENOLS. Journal of food biochemistry, 42(3), e12496.

+ Open protocol

+ Expand

Cell Cycle and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For cell cycle analysis, after 48 h of transfection, SGC-7901 and BCG-823 cells were digested with trypsin and washed with PBS, followed by staining with propidium iodide (PI) (Multi Sciences, China). The Annexin V/PI assay kit (556,547, BD Biosciences, CA, USA) was used to detect apoptosis. Cells were resuspended in 100 μL of binding buffer. Then 5 μL Annexin V-FITC and 5 μL PI staining solution were added, mixed well, and incubated for 15 min in the dark. Then, the cell cycle distribution and apoptosis were performed with flow cytometry (FACS Calibur, BD Bioscience, USA).

Zhang C., Wang H., Liu Q., Dai S., Tian G., Wei X., Li X., Zhao L, & Shan B. (2023). LncRNA CCAT1 facilitates the progression of gastric cancer via PTBP1-mediated glycolysis enhancement. Journal of Experimental & Clinical Cancer Research : CR, 42, 246.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!