Lipod293 in vitro dna transfection reagent

Manufactured by Addgene

LipoD293™ is an in vitro DNA transfection reagent. It is designed to facilitate the delivery of DNA into mammalian cells.

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Lab products found in correlation

Pmd2 g plasmid, by Addgene (2 mentions) Pspax2, by Addgene (2 mentions)

2 protocols using lipod293 in vitro dna transfection reagent

Lentiviral Vector Production in HEK293T Cells

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In 100 mm dishes, HEK293T cells were transfected using 6 μg of the proposed plasmid, 4 μg of psPAX2 (Addgene plasmid, # 12260) and 2 μg of pMD2.G plasmid (Addgene plasmid, #12259) per well with LipoD293™ In Vitro DNA Transfection Reagent. The transfected cells were incubated under normal conditions for 48 h and then harvested. The supernatant of HEK293T cell culture was collected after centrifugation at room temperature (RT). Concentration of virus was quantified using Lentivirus Concentration Solution (Genomeditech, #GM-040801-100) following manufacture's protocol. The lentivirus was stored at −80°C before use.

Xia Z., Tang M., Ma J., Zhang H., Gimple R.C., Prager B.C., Tang H., Sun C., Liu F., Lin P., Mei Y., Du R., Rich J.N, & Xie Q. (2021). Epitranscriptomic editing of the RNA N6-methyladenosine modification by dCasRx conjugated methyltransferase and demethylase. Nucleic Acids Research, 49(13), 7361-7374.

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HEK293T Cell Lentiviral Transfection

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In 100 mm dishes, HEK293T cells were transfected using 6 μg of the proposed plasmid, 4 μg of psPAX2 (Addgene plasmid # 12260), and 2 μg of pMD2.G plasmid (Addgene plasmid # 12259) per well with LipoD293™ In Vitro DNA Transfection Reagent. The transfected cells were incubated under normal conditions for 48 hours and then harvested. The supernatant of HEK293T cell culture was collected after centrifugation at room temperature (RT).

Xia Z., Tang M., Zhang H., Gimple R.C., Prager B.C., Tang H., Sun C., Liu F., Peng L., Mei Y., Du R., Rich J., & Xie Q. (2020). Epitranscriptomic editing of the RNA N6-methyladenosine modification by dCasRx conjugated methyltransferase and demethylase.

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