Beta actin 20536 1 ap

RIPA buffer (Sigma-Aldrich, USA) mixed with a protease inhibitor cocktail (Roche) were adopted to extract total proteins from samples and protein concentrations were examined by the bicinchoninic acid (BCA) solution (Pierce, USA). SDS-containing polyacrylamide gel (SDS-PAGE) was conducted to separate cell protein lysates, which were transmitted to 0.22 mm polyvinylidene fluoride membranes (PVDF) membrane (Bio-Rad, USA) and investigated with specific antibodies. EPHB3 (PA5-82337) were purchased from Invitrogen (USA); GM130 (ab52649), p62 (ab56416), CD63 (ab217345), and Alix (ab88388) were purchased from Abcam (USA); LC3 (#12741) was purchased from Cell Signaling Technology (USA); Beta Actin (20536-1-AP) was purchased from Proteintech (China). The standard WB was conducted under the primary antibodies and secondary antibodies.

CTN (MSS1009) was provided by Pribolab (Qingdao, Shandong, China). 4-PBA was purchased from Sigma Commercial (St Louis, MO, USA). Glutathione (GSH) (A006-2-1), malondialdehyde (MDA) (A003-1-2), superoxide dismutase (SOD) (A001-3-2), catalase (CAT) (A007-1-1), nitric oxide (NO) (A013-2-1), calcium ion (C004-2-1), and bicinchoninic acid (BCA) assay kits (A045-4-2) were purchased from Nanjing Jian Cheng Bioengineering Institute (Nanjing, Jiangsu, China). Hematoxylin and eosin (H&E) staining solution (G1005) was purchased from Wuhan Servicebio (Wuhan, Hubei, China). Hypersensitive ECL chemiluminescence Kit (P10100) was purchased from NCM Biotech (Suzhou, Jiangsu, China). Bax (380709) and Bcl-2 (381702) antibodies were provided by Chengdu Positive Energy biology (Chengdu, China). The Caspase-12 (35965S) antibody was provided by Cell Signaling Technology Inc. (Danvers, MA, USA); CHOP (15204-1-AP), GRP78 (11587-1-AP), Caspase-3 (19677-1-AP), and Beta Actin (20536-1-AP) antibodies were provided by Proteintech (Wuhan, China). Therm (Fementas) offered the protein marker (26616) (Burlington, VT, USA).