Endocab iga

EndoCAb IgG, EndoCAb IgM, and EndoCAb IgA were measured using direct ELISA kits procured from Hycult Biotech. LBP and LL-37 were measured using sandwich ELISA kits procured from Hycult Biotech (Pennsylvania, USA). sCD14 and FABP2 were measured using sandwich ELISA kits from R&D systems (Minneapolis, USA). CXCL16 was analyzed using a sandwich ELISA kit procured from Thermo Scientific (Frederick, MD, USA). Galectin-3 was measured using sandwich ELISA kit (Abcam, MA, USA). Total IgG, IgA, and IgM were measured using sandwich ELISA kits procured from Invitrogen (Carlsbad, CA, USA). The samples were diluted in appropriate buffers, which contains HeteroBlock (Omega Biologicals, Bozeman, MT, USA) to block non-specific antibodies which may interfere with the assay. All the analyses were performed blinded to clinical status with the exception of galectin-3 and total immunoglobulins IgG, IgA, and IgM.

sCD14 and LBP were measured using sandwich ELISA kits procured from R&D systems (Minneapolis, USA) and Hycult Biotech (Pennsylvania, USA), respectively. EndoCAb IgG, EndoCAb IgA, and EndoCAb IgM were measured using direct ELISA kits procured from Hycult Biotech. CXCL16 was analyzed using a sandwich ELISA kit procured from Thermo Scientific (Frederick, MD, USA). Lysozyme levels were measured using sandwich ELISA kit procured from MBL (Massachusetts, USA). Total IgG, IgA, and IgM were measured using sandwich ELISA kits procured from Invitrogen (Carlsbad, CA, USA). To block non-specific antibodies that may interfere with the assay, the samples were diluted in appropriate buffers, which contained 50 μg/ml of HeteroBlock (Omega Biologicals, Bozeman, MT, USA) and kept for 30 minutes before adding into the ELISA plate. All the analyses were performed blinded to case/control and clinical status. In order to maintain the test quality and reproducibility, an internal control was included in all the assays and the coefficient of variation (CV) of replicates was set at ≤ 10%.