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2 protocols using trichloromethane chcl3

1

Formulation and Evaluation of CBZ-Loaded Liposomes

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Distearoyl-sn-glycerol-3-phosphoethanolamine-N-[maleimide(polyethyleneglycol)–2000] (DSPE-PEG2000), egg phosphatidyl lipid-80 (E80), and cholesterol were purchased from Lipoid GmbH (Ludwigshafen, Germany). CBZ was purchased from Taxus Co, Ltd. (Jiangsu, China). 6-coumarin, 3-(4, 5-dimethy lthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT reagent), and DAPI were purchased from Sigma (St Louis, USA). EthD-1 and Calcein AM (Live/Dead Viability/Cytotoxicity Kit) were acquired from Life Technologies (Carlsbad, CA). FITC-Annexin V/PI apoptosis kit was provided by MultiSciences Biotech (Zhejiang, China). RPMI 1640 medium (RPMI), Dulbecco's modified Eagle's medium, fetal bovine serum (FBS), and penicillin / streptomycin (100 U/ml) were purchased from JiNuo Biotechnology Co., Ltd. (Zhejiang, China). Dimethyl sulfoxide (DMSO), methanol, and trichloromethane (CHCl3) were obtained from Sinopharm Chemical Reagent Co. All other chemicals were of analytical grade and used without further purification. The deionized water used in all experiments was prepared using a Milli-Q system (Millipore, Boston).
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2

Multifunctional Nanoparticle-Mediated Immunotherapy

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Dipalmitoyl phosphatidylcholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)2000] (DSPE-PEG-2000), Cy5.5-DSPE-PEG and cholesterol were purchased from Xi’an Ruixi Biological Technology Co., Ltd. Imiquimod (R837) was purchased from Sigma-Aldrich. Hematoporphyrin monomethyl ether (HMME) was provided by Di-Bai Chemical Technology Co., Ltd. Dimethyl sulfoxide (DMSO), methanol and trichloromethane (CHCl3) were purchased from the Sinopharm Chemical Reagent Co. Anti-PD-L1 was obtained from Bioxcell (α-PD-L1, Clone: 10 F.9G2, Catalog No. BE0101). ELISA kit was purchased from R&D Systems, USA.
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