Filter
paper cards used
were Whatman 903 Protein Saver cards (GE Healthcare Life Sciences,
Chicago, IL, USA). A manual puncher from McGill (Advantus Corp., Jacksonville,
FL, USA) was used to punch the DBS. Microtubes were obtained from
Sarstedt (Nümbrecht, Germany). For extraction, a Thermomixer
Comfort (Eppendorf, Hamburg, Germany) was used. Glass tubes for evaporation
to dryness were obtained from VWR (Radnor, PA, USA), and the evaporator
used was a TurboVap LV (Caliper Life Sciences, Waltham, MA, USA).
HPLC vials, caps, and inserts were from La-Pha-pack (Thermo Scientific,
Waltham, MA, USA).
The following LC columns were evaluated:
Polaris C18-Ether and Pursuit XRs Diphenyl (both from Agilent Technologies
(Santa Clara, CA, USA)), C18-Pentafluorophenyl (PFP) from ACE Technologies
(Aberdeen, Scotland), Aeris Peptide XB-C18 (Phenomenex (Torrance,
CA, USA)), and Raptor Biphenyl (Restek (Bellefonte, PA, USA)). For
column specifications, seeTable 1 .
paper cards used
were Whatman 903 Protein Saver cards (GE Healthcare Life Sciences,
Chicago, IL, USA). A manual puncher from McGill (Advantus Corp., Jacksonville,
FL, USA) was used to punch the DBS. Microtubes were obtained from
Sarstedt (Nümbrecht, Germany). For extraction, a Thermomixer
Comfort (Eppendorf, Hamburg, Germany) was used. Glass tubes for evaporation
to dryness were obtained from VWR (Radnor, PA, USA), and the evaporator
used was a TurboVap LV (Caliper Life Sciences, Waltham, MA, USA).
HPLC vials, caps, and inserts were from La-Pha-pack (Thermo Scientific,
Waltham, MA, USA).
The following LC columns were evaluated:
Polaris C18-Ether and Pursuit XRs Diphenyl (both from Agilent Technologies
(Santa Clara, CA, USA)), C18-Pentafluorophenyl (PFP) from ACE Technologies
(Aberdeen, Scotland), Aeris Peptide XB-C18 (Phenomenex (Torrance,
CA, USA)), and Raptor Biphenyl (Restek (Bellefonte, PA, USA)). For
column specifications, see