The largest database of trusted experimental protocols

Accunanobead streptavidin magnetic nanobeads

Manufactured by Bioneer

AccuNanoBead™ Streptavidin Magnetic Nanobeads are uniform, superparamagnetic nanoparticles coated with streptavidin. They are designed for efficient capture and separation of biotinylated molecules.

Automatically generated - may contain errors

2 protocols using accunanobead streptavidin magnetic nanobeads

1

Affinity Purification of Myc-tagged Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
pCMV–Myc–ATP synthase 5 alpha, pCMV–Myc–ATP synthase 5 beta, or pCMV–Myc–14–3–3ζ plasmids were transfected into HEK293T cells (CRL–11268; ATCC). Transfected cells were selected with 3 μM puromycin (BML-GR312-0050; Enzo Life Sciences, Farmingdale, NY, USA) for 14 days. During selection, the medium was changed every 4 day. After selection, cells were treated with 8 μM biotinylated KB1541 for 5 days. Cells were lysed with lysis buffer [50 mM Tris-HCl (pH 7.4), 150 mM KCl, 1 mM PMSF, 2 mM benzamidine, 0.05% NP-40, protease inhibitor cocktail (04693116001; Roche)]. Then, precipitation was performed using AccuNanoBead™ Streptavidin Magnetic Nanobeads (TA-1015-1; Bioneer). Proteins were separated on a 10% SDS–PAGE gel and analyzed by immunoblotting.
+ Open protocol
+ Expand
2

Biotinylated KB1541 Interactome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Senescent fibroblasts were treated for 14 days with 8 μM biotinylated KB1541. Immunoprecipitation was performed using AccuNanoBead™ Streptavidin Magnetic Nanobeads (TA-1015-1; Bioneer, Daejon, Korea). The eluted proteins were applied on to an Ion Mobility Tandem Mass Spectrometer/Time-of-flight mass spectrometry (IM–MS/MS TOF) (LCQ Deca XP-Plus, Thermo Finnigan, San Jose, CA, USA). Data analysis was performed as previously described [54 (link)].
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!