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Recombinant lif

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Recombinant LIF is a cytokine that supports the self-renewal of embryonic stem cells. It is produced in a mammalian cell expression system.

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Recombinant human LIF

2 protocols using recombinant lif

1

HDAC Inhibitor Effects on LIF Signaling

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Cells were treated with vehicle (DMSO), entinostat (5μM) or panobinostat (50nM) for a total of 6 hours (MCF7) or 24 hours (MDA-MB-231b). Recombinant LIF (R&D Systems, 50 ng/ml−1) or vehicle (0.1% BSA+PBS) was added to the medium for the final 15 minutes of HDACi treatment and protein harvested as discussed above. All treatments were performed in full serum medium.
Clements M.E., Holtslander L., Edwards C., Todd V., Dooyema S.D., Bullock K., Bergdorf K., Zahnow C.A., Connolly R.M, & Johnson R.W. (2021). HDAC inhibitors induce LIFR expression and promote a dormancy phenotype in breast cancer. Oncogene, 40(34), 5314-5326.
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2

HDAC Inhibitor Effects on LIF Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with vehicle (DMSO), entinostat (5μM) or panobinostat (50nM) for a total of 6 hours (MCF7) or 24 hours (MDA-MB-231b). Recombinant LIF (R&D Systems, 50 ng/ml−1) or vehicle (0.1% BSA+PBS) was added to the medium for the final 15 minutes of HDACi treatment and protein harvested as discussed above. All treatments were performed in full serum medium.
Clements M.E., Holtslander L., Edwards C., Todd V., Dooyema S.D., Bullock K., Bergdorf K., Zahnow C.A., Connolly R.M, & Johnson R.W. (2021). HDAC inhibitors induce LIFR expression and promote a dormancy phenotype in breast cancer. Oncogene, 40(34), 5314-5326.
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