4800 plus proteomics analyzer maldi tof ms

Chemicals and reagents were purchased from Sigma-Aldrich (Saint Louis, MO) except where indicated. Dynabeads^® MyOne^™ Tosyl-activated magnetic beads were purchased from Invitrogen Co. (Carlsbad, CA). Recombinant LF (rLF) was purchased from List Biological Laboratories (Campbell, CA). Two non-neutralizing monoclonal anti-LF antibodies (LF-mAb) were prepared in the Division of Scientific Resources of CDC as previously described [19 (link)]. AIGIV was obtained from the Division of Strategic National Stockpile, CDC (Atlanta, GA). Pooled human donor plasma (n = 10 individuals per pool) (PH-plasma) and individual normal North American (NNA) donor serum and plasma samples were obtained from Interstate Blood Bank (Memphis, TN). Equipment included a Kingfisher 96 magnetic particle processor (Thermo Fisher Scientific., Waltham, MA), 4800 Plus Proteomics Analyzer (MALDI-TOF MS) (AB Sciex, Foster City, CA) and Gene Amp^@ PCR thermocycler (ThermoFisher, Waltham, MA). All procedures and sample handling were conducted at Biosafety Level 2 containment as recommended and described [21 ]. Additional safety measures are described in each section as needed.