For all the animal experiments in the present study, the study protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of UT MD Anderson Cancer Center. Male BALB/c nude mice, male NOD SCID gamma mice, male C57BL/6J mice, and male FVB/NJ mice (aged 4–6 weeks) were obtained from The Jackson Laboratory. For primary tumor growth assays, cells were resuspended in 100 μL PBS with Matrigel in 1:1 ratio and subcutaneously injected into both rear flanks. The volume of the s.c. xenograft was calculated as V = L × W2/2, where L and W stand for tumor length and width, respectively. For experimental metastasis assays, cells were resuspended in 100 μL PBS and injected into the left ventricle with a 26G tuberculin syringe. For drug treatment, drug solutions were delivered either intraperitonially or by oral gavage using a 20G reuseable feeding needle (Roboz Surgical Co.). Metastatic burden was detected through noninvasive bioluminescence imaging of experimental animals using an IVIS Spectrum and Biospec 7T MRI instruments at the Small Animal Imaging Facility (SAIF). To investigate the effect of drug treatment, compounds were delivered daily through p.o. Bioluminescence signals were measured using the ROI tool in Living Image software (Xenogen).
Nod scid gamma male mice
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
NOD/SCID gamma male mice are a strain of immunodeficient mice characterized by the absence of mature T cells, B cells, and functional natural killer cells. These mice are commonly used in biomedical research as a model for studying human diseases and evaluating experimental therapies.
Automatically generated - may contain errors
Lab products found in correlation
Male fvb nj mice, by Jackson ImmunoResearch (2 mentions)
Male balb c nude mice, by Jackson ImmunoResearch (2 mentions)
C57bl 6j male mice, by Jackson ImmunoResearch (2 mentions)
Ivis instrument, by PerkinElmer (2 mentions)
Luciferin, by PerkinElmer (2 mentions)
Nanozoomer digital pathology software, by Hamamatsu Photonics (1 mentions)
Nanozoomer slide scanner, by Hamamatsu Photonics (1 mentions)
9 protocols using nod scid gamma male mice
1
Preclinical In Vivo Cancer Models
2
Preclinical In Vivo Cancer Models
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For all the animal experiments in the present study, the study protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of UT MD Anderson Cancer Center. Male BALB/c nude mice, male NOD SCID gamma mice, male C57BL/6J mice, and male FVB/NJ mice (aged 4–6 weeks) were obtained from The Jackson Laboratory. For primary tumor growth assays, cells were resuspended in 100 μL PBS with Matrigel in 1:1 ratio and subcutaneously injected into both rear flanks. The volume of the s.c. xenograft was calculated as V = L × W2/2, where L and W stand for tumor length and width, respectively. For experimental metastasis assays, cells were resuspended in 100 μL PBS and injected into the left ventricle with a 26G tuberculin syringe. For drug treatment, drug solutions were delivered either intraperitonially or by oral gavage using a 20G reuseable feeding needle (Roboz Surgical Co.). Metastatic burden was detected through noninvasive bioluminescence imaging of experimental animals using an IVIS Spectrum and Biospec 7T MRI instruments at the Small Animal Imaging Facility (SAIF). To investigate the effect of drug treatment, compounds were delivered daily through p.o. Bioluminescence signals were measured using the ROI tool in Living Image software (Xenogen).
3
Hepatoma Tumor Growth in NSG Mice
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Male NOD.scid.gamma (NSG) mice (8-12 wk old) purchased from the Jackson Labs (Bar Harbor, ME, United States) were used to evaluate tumor growth in vivo under protocols approved by the Université de Sherbrooke ethical committee on animal care and use. To evaluate the growth of hepatoma cells in the liver, cells were injected via intravenous or intrasplenic/portal route. For intravenous inoculation, 106 Hepa-vector or Hepa-SOCS1 cells in 100 μL volume were injected via the caudal vein. For intrasplenic inoculation, mice were anesthetized with ketamine (10 mg/kg) and the spleen was exposed through a small abdominal incision[24 (link)]. Tumor cells (106 cells in 100 μL) were injected into the spleen and mice were splenectomized 2 min later. Tumor nodules in the liver were examined 20 d later when the animals began to show distress. The images of hematoxylin and eosin (H and E) -stained sections of the liver were acquired using Nanozoomer Slide Scanner and analyzed by the Nanozoomer Digital Pathology (NDP) software (Hamamatsu Photonics, Japan).
4
PDX Xenograft of Prostate Cancer in NSG Mice
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Male NOD-SCID-gamma (NSG) mice (Jackson Labs), 6–12 weeks old were used. Human derived PDX (patient derived xenografts) tissues from prostatectomies, were resected, and necrotic tissues removed and mechanically sectioned into smaller fragments (~1–2 mm3 per graft size) and subsequently these were xenografted into the sub-renal capsule. Mice were treated 1 week after with enzalutamide daily for 4 consecutive days at 1 mg/mouse by oral gavage. These studies were conducted according to the Cedars-Sinai Institutional Animal Care & Use Committee (CSMC IACUC) #IACUC007440.
5
De novo BPDCN Mouse Model
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Male NOD SCID Gamma (NSG) mice at 7 to 8 weeks old purchased from The Jackson Laboratory were used for CAL-1 xenograft experiments. TET2 Δ/Δ P53 Δ/Δ DKO + MYC RUNX2 OE, a de novo BPDCN mouse model was generated by Kubota and colleagues (2019).5 (link) RNA-sequencing (RNA-seq) data obtained from leukemic cells from this mouse model were used for gene expression analyses.
6
Tumor Growth in NOD/SCID Mice
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All animal studies were performed in accordance with a protocol approved by the Academia Sinica Institutional Animal Care and Utilization Committee. Age-matched severe combined immune deficiency mutation and IL2 receptor gamma chain deficiency (NOD/SCID gamma) male mice (6–8 weeks old), originally from The Jackson Laboratory, were used in this study. To estimate in vivo tumor growth, 1 × 103 and 1 × 104 cancer stem cells were resuspended in 0.1 ml of PBS and injected into the left flank of mice (n = 5). Randomize mice for animal experiments in these manuscript.
7
Melanoma Growth Regulated by SPRY4
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Sixteen Nod-SCID-gamma male mice of 6-week-old (Jackson Laboratory, Bar Harbor, ME) were divided into two groups, eight mice per group were injected subcutaneously (s.c.) with 0.2 million SK-MEL-119NRASQ61R cells constitutively expressing either control CD516B2-vector or CD516B2-SPRY4 in 0.1 ml 10% DMEM growth medium per flank of mice. Animals were monitored twice weekly for 7 weeks. Body weights and tumor size were measured every week as described previously [27 (link)]. Data were expressed as mean ± SD, N = 8. Tumor histology was confirmed by hematoxylin/eosin staining of formalin-fixed and paraffin-embedded tissue. Animals were maintained in well-ventilated animal facility and tested in accordance with the MGH Animal Care and Use Committee guidelines.
8
Xenograft Tumor Establishment and In Vivo Imaging
9
Xenograft Tumor Induction and Imaging
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All animal studies were performed according to IACUC guidelines. NOD/SCID gamma male mice (The Jackson Laboratory), aged 8 to 10 weeks, were used in all experiments. For splenic (portal circulation) injections, cells were injected directly into the spleen followed by splenectomy (250k for SW480 and LS lines and 100k for WiDr cells). In vivo bioluminescence was measured by retro-orbital injection of luciferin (Perkin Elmer) followed by imaging with an IVIS instrument (Perkin Elmer). For ex vivo liver imaging, mice were injected with luciferin prior to liver extraction, and the liver was then imaged and weighed after rinsing with PBS.
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