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Hpsec

Manufactured by GE Healthcare
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Sourced in United States

HPSEC is a laboratory equipment used for the analysis and separation of macromolecules such as proteins, polymers, and other high-molecular-weight compounds. It utilizes high-performance size-exclusion chromatography to separate molecules based on their size and molecular weight.

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Lab products found in correlation

Dextrans, by Merck Group (2 mentions) Tsk gel 3000, by GE Healthcare (2 mentions)

2 protocols using hpsec

1

Determining Molecular Weight by HPSEC

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The apparent molecular weight of samples was determined by high-performance size exclusion chromatography (HPSEC) (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) on TSK-Gel® 3000 (30 123 cm × 0.75 cm), with a column temperature of 30 °C. The samples were eluted with ultrapure water and 0.1 M NaNO2 acetate buffer, at a flow rate of 0.6 mL/min and detected by a refractive index detector. The column was calibrated using different dextrans (10; 47; 74; 147 kDa) purchased from Sigma (St. Louis, MO, USA).
Fernandes-Negreiros M.M., Batista L.A., Silva Viana R.L., Araujo Sabry D., Paiva A.A., Paiva W.S., Machado R.I., de Sousa Junior F.L., de Lima Pontes D., Vitoriano J.D., Alves Junior C., Lanzi Sassaki G, & Rocha H.A. (2020). Gallic Acid-Laminarin Conjugate Is a Better Antioxidant than Sulfated or Carboxylated Laminarin. Antioxidants, 9(12), 1192.
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2

Chitosan Molecular Weight Analysis

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The molecular weight of chitosan was determined by high performance size exclusion chromatography (HPSEC) (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) on TSK-Gel® 3000 (30 cm × 0.75 cm), with a column temperature of 60 °C. The chitosan was eluted with 0.2 M NaCl in 0.05 M acetate buffer, pH 5.0, at a flow rate of 1.0 mL/min and detected by a refractive index detector. The column was calibrated using different dextrans (10; 47; 74; 147 kDa) purchased from Sigma (St. Louis, MO, USA).
Queiroz M.F., Melo K.R., Sabry D.A., Sassaki G.L., Rocha H.A, & Costa L.S. (2019). Gallic Acid-Chitosan Conjugate Inhibits the Formation of Calcium Oxalate Crystals. Molecules, 24(11), 2074.
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