Quantum gx μct

Ninj1^−/− mice were described previously^{18 (link)}. All animal studies were performed with male mice to exclude sex hormonal effects. Bone marrow cells were obtained from femurs and tibias of 7-week-old Ninj1^−/− mice or their wild-type (WT) littermates and used for differentiation into macrophages or OCs. Hind limbs isolated from 6- or 12-week-old mice were scanned individually with a radiology apparatus. Plain radiologic and μCT images were obtained with an IVIS Spectrum CT (PerkinElmer, Waltham, MA) and Quantum GX μCT (PerkinElmer) at 10-μm isotropic resolution, respectively. Trabecular bone samples with lengths of over 1 mm encompassing the region of the distal metaphysis away from the proximal edge of the growth plate in the distal end of femurs were analyzed using Inveon Research Workplace (Siemens, Malvern, PA).
All mice were housed in the animal care facility of Seoul National University under specific pathogen-free conditions, and all animal procedures were approved by the Institutional Animal Care and Use Committees of Seoul National University.

The right femurs were subjected to the μ-CT scanning as previously described [36 (link),37 (link)]. Briefly, the right femur was scanned and captured by the Quantum GX μCT instrument (PerkinElmer, Waltham, MA, USA). The parameters in the volume of interest were analyzed by the Analyzer Software (V12.0), including (1) BMD (bone mineral density); (2) BV/TV (bone volume fraction); (3) BS/TV (bone surface density); (4) Tb.N (trabecular number); (5) Tb.Sp (trabecular separation); (6) Tb.Th (trabecular thickness); (7) Conn.D (connectivity density); and (8) SMI (Structure Model Index).