HCC cell lines (HepG2, Hep3B, SNU-423, and PLC/PRF/5) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The human normal liver L02 cell line and Huh-7, MHCC-LM3, and MHCC97-H cells were obtained from CELLCOOK (Guangzhou, China) and cultured with 5% CO2 at 37°C. The cells were maintained in high-glucose Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and passaged every 3 days.
Mhcc97 h
Manufactured by Cellcook
Sourced in China
The MHCC97-H is a laboratory equipment used for cell culture research. It is designed to maintain optimal environmental conditions for the growth and maintenance of cell lines. The core function of the MHCC97-H is to provide a controlled and stable environment for cell culture experiments.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Hepg2, by American Type Culture Collection (2 mentions)
Hep3b, by American Type Culture Collection (2 mentions)
Plc prf 5, by American Type Culture Collection (2 mentions)
Mhcc lm3, by Cellcook (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Snu423, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Sk hep1, by American Type Culture Collection (1 mentions)
Thle 3, by American Type Culture Collection (1 mentions)
Thle 3, by Lonza (1 mentions)
Begm medium, by Lonza (1 mentions)
Mhcc 97l, by Cellcook (1 mentions)
Fetal bovine serum (fbs), by Beckman Coulter (1 mentions)
Optima l 100 xp, by Beckman Coulter (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Huvecs, by ScienCell (1 mentions)
Endothelial cell medium (ecm), by ScienCell (1 mentions)
4 protocols using mhcc97 h
1
Culturing Hepatocellular Carcinoma Cell Lines
2
Characterization of Human Liver Cancer Cell Lines
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Human HCC cell lines (Hep 3B and SK-HEP-1), human normal hepatocyte cell line (THLE-3), and human embryonic kidney cell line (HEK293T) were acquired from American Type Culture Collection (ATCC; Manassas, VA, USA). Human HCC cell line (Huh7) was derived from Chinese Academy of Sciences (Shanghai, China). Human HCC cell line (HCCLM3) was obtained from Procell Life Science & Technology (Wuhan, China). Human HCC cell line (MHCC97-H) was purchased from Cellcook (Guangzhou, China). THLE-3 cells were cultured in bronchial epithelial growth medium (BEGM) medium (LONZA, Basel, Switzerland) with 10% FBS (Thermo Fisher Scientific, Waltham, MA, USA). Other cells were incubated in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO, Rockville, MD, USA) adding 10% FBS plus 1% penicillin/streptomycin (Thermo Fisher Scientific). All media were utilized for cell culture at 37°C with 5% CO2.
3
Culturing Human Liver Cell Lines
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The American Type Culture Collection (ATCC, Manassas, VA, USA) supplied the HepG2, 293T, PLC/PRF/5, and Hep3B cell lines. Normal human liver cell lines L02, MIHA, QGY, MHCC-LM3, MHCC97-H, MHCC97-L, and Huh-7 were purchased from Cellcook Biotech (Guangzhou, China). We cultured the cells in Dulbecco's modified Eagle's medium (DMEM) with 5% CO2 at 37 °C, which contains 10% FBS and 1× streptomycin-penicillin. Cells were passaged at 80% confluence.
4
Establishing ANGPT2-Overexpressing and -Knockdown HCC Cell Lines
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Hep3B, SNU182, SNU387 and Li7 cells were kindly provided by Stem Cell Bank, Chinese Academy of Sciences (China). MHCC97H and HUVEC cells were purchased from Guangzhou Cellcook Biotech Co., Ltd. (China). Five HCC cell lines, Hep3B, SNU182, SNU387, Li7 and MHCC97H, were cultured in Roswell Park Memorial Institute 1640 medium (RPMI 1640, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA). HUVECs were cultured in endothelial cell medium (ECM, ScienCell, USA). All cells were maintained in a humidified incubator at 37 °C with 5% CO2. The FBS used for exosome isolation was depleted of exosomes by ultracentrifugation for 12 h at 120,000 x g at 4 °C (Optima L-100XP, Beckman, USA). The following sublines were established by infecting cells with the lentiviruses pLV-hANGPT2-mCherry, pLV-mCherry, lentiCRISPRv2-ANGPT2gRNA or lentiCRISPRv2: Hep3B and MHCC97H sublines that stably overexpressed ANGPT2 (named Hep3B-ANGPT2 and MHCC97H-ANGPT2, respectively) and their matched control lines (named Hep3B-CT and MHCC97H-CT, respectively), and those that stably knocked down ANGPT2 (named Hep3B-ANGPT2crispr and MHCC97H-ANGPT2crispr, respectively) and their matched control lines (named Hep3B-V2 and MHCC97H-V2, respectively).
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