Mmp 9 elisa kit

Manufactured by R&D Systems

Sourced in United States

The MMP-9 ELISA kit is a quantitative immunoassay designed for the measurement of MMP-9 levels in biological samples. The kit utilizes the sandwich ELISA technique and contains the necessary components to perform the assay.

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Lab products found in correlation

Gapdh, by Cell Signaling Technology (2 mentions) Spectramax i3, by Molecular Devices (1 mentions) S100a8 a9 elisa kit, by R&D Systems (1 mentions) Type 2 collagenase solution, by Merck Group (1 mentions) Sc 53534, by Santa Cruz Biotechnology (1 mentions) Ab39012, by Abcam (1 mentions) Ab124824, by Abcam (1 mentions) Ab38898, by Abcam (1 mentions) Ab170099, by Abcam (1 mentions) Dve00, by R&D Systems (1 mentions) Novex zymogram gel, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Molecular Devices (1 mentions) Bp121s, by Sartorius (1 mentions) Rabbit anti caspase 3, by Cell Signaling Technology (1 mentions) Cell culture flask, by Corning (1 mentions) Uv imaging system, by Analytik Jena (1 mentions) Rabbit anti β catenin, by Cell Signaling Technology (1 mentions) Pipettor, by Eppendorf (1 mentions) Rabbit anti gsk 3β, by Cell Signaling Technology (1 mentions) Rabbit anti pgsk3β ser9, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

ELISAs (144 citations) MMP-9 (142 citations)

15 protocols using mmp 9 elisa kit

Quantify MMP9 in M0-CM by ELISA

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MMP9 concentrations in M0-CM were determined using a commercially available MMP9 ELISA kit (DY911–05, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Tekin C., Aberson H.L., Waasdorp C., Hooijer G.K., de Boer O.J., Dijk F., Bijlsma M.F, & Spek C.A. (2020). Macrophage-secreted MMP9 induces mesenchymal transition in pancreatic cancer cells via PAR1 activation. Cellular Oncology (Dordrecht), 43(6), 1161-1174.

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VEGF and MMP-9 Quantification in CM

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The concentrations of VEGF and MMP-9 in the CM were detected using a VEGF ELISA kit (cat. no. DVE00; R&D Systems, Inc.) and an MMP-9 ELISA kit (cat. no. DMP900; R&D Systems, Inc.), respectively, following the manufacturer's protocol. The absorbance was measured at 450 nm using a microplate reader (SpectraMax i3; Molecular Devices, LLC).

Okikawa S., Morine Y., Saito Y., Yamada S., Tokuda K., Teraoku H., Miyazaki K., Yamashita S., Ikemoto T., Imura S, & Shimada M. (2022). Inhibition of the VEGF signaling pathway attenuates tumor-associated macrophage activity in liver cancer. Oncology Reports, 47(4), 71.

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ELISA Validation of Lung Proteins

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Lung of six mice were used for Elisa validation. Three mice in control group and the other three in infection group. The MMP-9 Elisa kit (R&D, MMPT90, the detection range was 0.08∼5 pg/mL) and S100a8/a9 Elisa kit (R&D, DY8596-05, the detection range was 125∼5,000 pg/mL) were used according to the manufacturer’s instructions to determine the concentration of proteins in the tissue homogenate. The proinflammatory factor IL-6 (R&D, DY406-05, the detection range was 15.6∼1,000 pg/mL) was also determined to show the host inflammatory response.

Li X., Liu X., Horvatovich P., Hu Y, & Zhang J. (2021). Proteomics Landscape of Host-Pathogen Interaction in Acinetobacter baumannii Infected Mouse Lung. Frontiers in Genetics, 12, 563516.

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RJNTF Therapeutic Pathway Modulation

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RJNTF was provided by the Third People’s Hospital of Fujian University of Traditional Chinese Medicine (The National Invention Patent has authorised the preparation method and use of this formula, Patent No. ZL201710284659.8 and ZL201810899834.9). Pentobarbital sodium (3%) was purchased from Fuchen Chemical Reagents Factory (Tianjin, China). Paraformaldehyde (4%) was purchased from Yongda Chemical Reagent Co., Ltd. (Tianjin, China). The ELISA kit was obtained from Cloud-Clone Corp (Wuhan, China). An immunohistochemical kit was collected from Boster Bioengineering Co., Ltd. (Wuhan, China). The MMP-9 ELISA kit was purchased from R&D Systems (MN, USA). Type II collagenase solution (0.2%) was obtained from Sigma-Aldrich (MO, USA). Sodium penicillin was purchased from Hycione (USA). The primary antibodies against CXCR4 (ab124824), MMP-3 (ab25915), MMP-9 (ab38898), MMP-13 (ab39012), and p38 (ab170099) were purchased from Abcam (USA). The primary antibodies against Anti-rabbit IgG antibody (#14708), Anti-mouse IgG antibody (#4408), p-p38 (#4511), and GAPDH (#5174) were obtained from Cell Signalling Technology (USA). The antibody against vinculin (26520-1-AP) was purchased from Proteintech (USA). The antibody against CXCR4 (sc-53534) was obtained from Santa Cruz Biotechnology (USA).

Chen J., Chen N., Zhang T., Lin J., Huang Y, & Wu G. (2022). Rongjin Niantong Fang ameliorates cartilage degeneration by regulating the SDF-1/CXCR4-p38MAPK signalling pathway. Pharmaceutical Biology, 60(1), 2253-2265.

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Serum Biomarkers for Surgical Patients

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Early in the morning before surgery, 8 mL of fasting peripheral blood was collected from the subjects and centrifuged at 2000 g for 10 min. The separated serum was stored in a -80°C freezer for subsequent measurement. The levels of S100A11 and MMP9 in peripheral blood were measured using the MMP-9 ELISA kit from R&D (USA) and the S100A11 ELISA kit from BioVendor.

Li W., Cui Z., Kong Y., Liu X, & Wang X. (2021). Serum Levels of S100A11 and MMP-9 in Patients with Epithelial Ovarian Cancer and Their Clinical Significance. BioMed Research International, 2021, 7341247.

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Serum Biomarker Preparation and Analysis

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A whole blood sample was held for 30 min at room temperature to allow clotting. The sample was centrifuged at 3,000 g for 10 min at 4 °C. The serum was transferred in separate tubes without disturbing blood clots and stored at −80 °C [13 (link)]. Full automatic biochemical analyzer (FABA) (HITACHI 7020) was used to assess total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C). The double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method was used to determine the concentration of adiponectin and MMP-9 in the serum. An enzyme-labeled instrument (Bio-Rad Benchmark Plus), blood parameter measurement reagent (Roche Diagnostics (Shanghai) Ltd. Shanghai, China), adiponectin ELISA kit (America Biomerica Company) and MMP-9 ELISA kit (USA & Canada | R&D Systems, Inc) were used in the experiments.

Wang X., Chen Q., Pu H., Wei Q., Duan M., Zhang C., Jiang T., Shou X., Zhang J, & Yang Y. (2016). Adiponectin improves NF-κB-mediated inflammation and abates atherosclerosis progression in apolipoprotein E-deficient mice. Lipids in Health and Disease, 15, 33.

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VEGF and MMP9 Secretion Assay

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ESCs were cultured and divided into three groups as described previously. Expression levels of VEGF and MMP9 secreted into the conditioned media derived from treated and untreated cells was determined according to manufacturer guidelines using VEGF (DVE00) and MMP9 Elisa kit (DMP900) from R&D Systems Minnesota, USA. All samples were assayed in duplicate. The amount of protein secreted was determined as an optical-density value using a microplate reader at a wavelength of 450 nm, with the correction wavelength set at 570 nm. A standard-curve analysis was included on each plate, and protein secretion was compared against this curve.

Zhang L., Xiong W., Li N., Liu H., He H., Du Y., Zhang Z, & Liu Y. (2016). Estrogen stabilizes hypoxia inducible factor 1 α through G protein coupled estrogen receptor 1 in eutopic endometrium of endometriosis. Fertility and sterility, 107(2), 439-447.

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Quantifying MMP9 Secretion and Activity

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Culture supernatants were collected from MNC or MNC-QQ cells and stored at −80 °C until use. We quantified the MMP9 levels secreted from cells using MMP9 ELISA kit (R&D Systems, MN, USA) according to the manufacturer's protocol. MMP9 levels were quantified by inspecting the samples at 450 nm absorbance on a microplate reader (Molecular Devices, CA, USA). MMP activity was investigated by Novex® zymogram gel according to the manufacturer's instructions (Thermo Fisher Scientific, Waltham, MA, USA).

Nishikai-Yan Shen T., Kado M., Hagiwara H., Fujimura S., Mizuno H, & Tanaka R. (2021). MMP9 secreted from mononuclear cell quality and quantity culture mediates STAT3 phosphorylation and fibroblast migration in wounds. Regenerative Therapy, 18, 464-471.

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Osteosarcoma Cell Line 143B Cytotoxicity

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Osteosarcoma cancer cell line 143B (Cell Bank of the Chinese Academy of Sciences, Shanghai, China), methyl thiazolyl tetrazolium (MTT) and DMSO (both from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), ICA (Aladdin Shanghai Biochemical Technology Co., Ltd., Shanghai, China), TRIzol kit and reverse transcription kit (both from Invitrogen, Carlsbad, CA, USA), VEGA ELISA kit and MMP-9 ELISA kit (both from R&D Systems, Inc., Minneapolis, MN, USA), rabbit anti-DKK1, rabbit anti-caspase-3, rabbit anti-β-catenin, rabbit anti-GSK3β, rabbit anti-pGSK3β (Ser9) and GAPDH (all from Cell Signaling Technology, Inc., Danvers, MA, USA), ECI luminescent solution (Invitrogen), inversed fluorescent microscope (Thermo Fisher Scientific GmbH, Dreieich, Germany), cell culture flask (Corning Inc., Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), PCR instrument (Applied Biosystems, Foster City, CA, USA), UV imaging system (Biometra, Goettingen, Germany), and electronic balance (BP121S; Sartorius AG, Goettingen, Germany) were used in the study. Any other equipment and reagents were previously described in the relevant section.

Ren Y., Zhu F, & Liu Z. (2018). Inhibitory effect of icariin on osteosarcoma cell proliferation via the Wnt/β-catenin signaling pathway. Oncology Letters, 16(2), 1405-1410.

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Quantification of MMP-9 Levels

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MMP-9 level was measured in supernatants from cells treated with for 24 h. Protein levels in the supernatants were assayed using a MMP-9 ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA) following the manufacturer's instruction. Optical density was measured at 450 nm. MMP-9 concentration was calculated by comparing the data to the known standards for MMP-9 proteins.

Zhang Y., Zheng W., Shen K, & Shen W. (2018). ∆9-tetrahydrocannabinol inhibits epithelial-mesenchymal transition and metastasis by targeting matrix metalloproteinase-9 in endometrial cancer. Oncology Letters, 15(6), 8527-8535.

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