Anti human cd106 vcam 1 pe

Sodium butyrate, propionate, trichostatin A (TSA, a selective and reversible hydroxamate inhibitor of class I and II HDACs) (Hebbel et al., 2010 (link)), sodium β-hydroxybutyrate (SHB, antagonist for GPR41 receptor) (Kimura et al., 2011 (link)), and LPS (Escherichia coli 0111:B4) were purchased from Sigma-Aldrich, St. Louis, MO, United States. Sodium acetate was bought from Merck Millipore. A cytotoxicity detection kit (lactate dehydrogenase, LDH) was obtained from Roche. Human IL-6, IL-8 ELISA (enzyme-linked immunosorbent assay) kits, and calcein-AM were purchased from Invitrogen. Human recombinant TNFα, anti-human CD106 (VCAM-1) PE, and viability fixable dyes were bought from eBioscience. GLPG0974 (GLPG, antagonist of GPR43 receptor) (Namour et al., 2016 (link)) was obtained from Tocris Bioscience. Primary anti-GPR41 antibody, anti-GPR43 antibody, and an HDAC activity assay kit were purchased from Abcam. EGM-2 Bulletkit was purchased from Lonza (Switzerland).

Sodium butyrate, propionate, and LPS (Escherichia coli 0111:B4) were purchased from Sigma-Aldrich, St. Louis, MO, United States. Acetate was bought form Merck Millipore (Germany). Cell cytotoxicity detection kit (lactate dehydrogenase, LDH) was obtained from Roche (Switzerland). Human IL-6 and IL-8 ELISA (enzyme-linked immunosorbent assay) kits were purchased from Invitrogen (Netherlands). Human recombinant TNFα, anti-human CD54 (ICAM-1) PE, anti-human CD106 (VCAM-1) PE and viability fixable dyes were bought from eBioscience (Netherlands). EGM-2 Bulletkit was bought from Lonza (Switzerland).