All mouse procedures were approved by the University of Illinois at Chicago Animal Care Committee. HCC1806 (1 × 106), MCF7 (3 × 106), MCF7-EMPTY (5 × 106) and MCF7-PSAT1 (5 × 106) cells were injected into the fat pad of the #4 mammary glands of 6 to 8-week-old athymic nude-foxn1nu female mice (Envigo). Cell suspensions were injected in a volume of 50 μL growth factor-reduced Matrigel (Corning). Estrogen (E2) was administered via silastic capsules implanted subcutaneously as previously described (Molloy et al., 2014 (link)). Even though not required for HCC1806 tumor growth, E2 was provided for all experiments to maintain constant conditions. All tumors were monitored by caliper measurements over time and tumor volume was calculated using the formula ½ (width2 x length). Mice were euthanized according to institutional guidelines. Serine and glycine free (TD.160752) and control (TD.110839) diets were purchased from Envigo and diet formulations are listed as reported (Sullivan et al., 2019b (link)). Custom diets were administered five days after surgery and replaced at least weekly.
Nude foxn1nu female mice
Manufactured by Inotiv
Nude-foxn1nu female mice are a laboratory mouse model that is athymic and lacks a functional thymus gland. This results in a lack of mature T cells, leading to impaired cell-mediated immunity. The Nude-foxn1nu female mice are commonly used in research applications that require an immunodeficient model.
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Lab products found in correlation
2 protocols using nude foxn1nu female mice
1
Mammary Fat Pad Xenograft Mouse Model
2
Mammary Fat Pad Xenograft Mouse Model
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All mouse procedures were approved by the University of Illinois at Chicago Animal Care Committee. HCC1806 (1 × 106), MCF7 (3 × 106), MCF7-EMPTY (5 × 106) and MCF7-PSAT1 (5 × 106) cells were injected into the fat pad of the #4 mammary glands of 6 to 8-week-old athymic nude-foxn1nu female mice (Envigo). Cell suspensions were injected in a volume of 50 μL growth factor-reduced Matrigel (Corning). Estrogen (E2) was administered via silastic capsules implanted subcutaneously as previously described (Molloy et al., 2014 (link)). Even though not required for HCC1806 tumor growth, E2 was provided for all experiments to maintain constant conditions. All tumors were monitored by caliper measurements over time and tumor volume was calculated using the formula ½ (width2 x length). Mice were euthanized according to institutional guidelines. Serine and glycine free (TD.160752) and control (TD.110839) diets were purchased from Envigo and diet formulations are listed as reported (Sullivan et al., 2019b (link)). Custom diets were administered five days after surgery and replaced at least weekly.
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