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2 protocols using ferrous chloride

1

Antioxidant Properties of Chlorella and Krill Oil

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Commercial Chlorella Pyrenoidosa powder and Antarctic krill oil were obtained from Dalian Jianyang Biological Company (Dalian, China) and Liaoyu Group Co., Ltd. (Dalian, China), respectively. The neutral proteases (EINECS:253-457-5) and alkaline proteases (EINECS:232-752-2) were obtained from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Ferrous chloride and ascorbic acid were obtained from Damao Chemical Reagent Factory (Tianjin, China). Sodium dodecyl sulfate (SDS), linoleic acid (stored at −20 °C), 1,1,3,3-tetraethoxypropane, trichloroacetic acid, Thiobarbituric acid (TBA), Nile Red, and Nile Blue were bought from Aladdin Industrial Co., Ltd. (Shanghai, China). Ammonium thiocyanate was bought from Sinopharm Group Reagent (Shanghai, China). Trypsin-EDTA, fetal bovine serum (FBS), and DMEM cell culture medium were bought from Invitrogen (Carlsbad, CA, USA). A superoxide dismutase (SOD) assay kit and reactive oxygen species (ROS) assay kit were bought from Nanjing Jiancheng Bioengineering Research Institute (Nanking, China).
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2

Montmorillonite-Based Antimicrobial Composite Synthesis

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The materials used include montmorillonite (pharmaceutical grade, Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China), dodecyl trimethylammonium chloride (DTAC; 99%, Shanghai Aladdin Biochemical Technology Co., Ltd.), dodecyl dimethyl benzyl chloride ammonium chloride (DDBAC; 98%, Shanghai Aladdin Biochemical Technology Co., Ltd.), octadecyl trimethyl ammonium chloride (STAC; 98%, Shanghai Aladdin Biochemical Technology Co., Ltd.), anhydrous ethanol (analytical grade, Xilong Science Co., Ltd., Shantou, China), silver nitrate (analytical grade, Tianjin Damao Chemical Reagent Factory, Tianjin, China), phosphotungstic acid (analytical grade, Tianjin Damao Chemical Reagent Factory), potassium bromide (spectral grade, Shanghai Aladdin Biochemical Technology Co., Ltd.), natural tapioca starch (industrial grade, Guangxi Cenxi Triangle Starch Co., Ltd., Cenxi, China), glycerin (98%, Shanghai Aladdin Biochemical Technology Co., Ltd.), ferrous chloride (analytical grade, Tianjin Damao Chemical Reagent Factory), ferric chloride (analytical grade, Tianjin Damao Chemical Reagent Factory) and sodium hydroxide (analytical grade, Tianjin Damao Chemical Reagent Factory).
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