Spd m10avp dad detector

This analysis was performed to determine the molecular masses of positive ions detected and to identify the components present in FBuOH and its sub-fractions. The analyses were conducted using a 10AD-VP chromatographic system coupled with a Shimadzu SPD-M10AVP DAD detector (Shimadzu, Kyoto, Japan) and an Esquire 3,000 Plus-Ion Trap mass spectrometer (Bruker Daltonics, GmbH, Bremen, Germany) equipped with a 4,000 V capillary, a nebulizer set at 27 psi, a drying gas flux of 7 l/min, and a temperature of 320°C, in positive ion mode.

Phenolic profiles of OPE were determined by HPLC coupled to a diode array (HPLC–DAD). Standard calibration curves were prepared by using gallic, protocatechuic acid, catechin, p-hydroxybenzoic, syringic, elagic, m-coumaric, o-coumaric, myricetin, quercetin, kaempferol, hydroxytyrosol, tyrosol, and luteolin. The HPLC analysis was performed using a modified method [5 (link)]. The samples and stock solutions were filtered through a 0.45 µm membrane filter and analyzed in a Shimadzu HPLC system (LC-10AD vp pump, SPDM10A vp DAD detector, SIL-10AD vp autosampler, CTO-10AVP column oven, DGU-14A degasser, and SCL-10A system controller; Shimadzu Corp., Kyoto, Japan). Separations were performed at 30 °C on Agilent Eclipse XDB-C18 reversed-phase column (250 mm × 4.6 mm length, 5 μm particle size). The mobile phase contained solvent A (3% (v/v) acetic acid) and solvent B (methanol). A gradient elution was carried out as shown: 28% B (0–20 min), 28–30% B (21–50 min), 31–50% B (51–70 min), and 50–100% B (70–81 min) and at 90 min was returned to initial conditions. The flow rate was 0.8 mL/min. Chromatograms were recorded at 278 nm. Identification and quantitative analysis were made based on the retention times and external standard curves. The amounts of polyphenols were stated in μg/g of dried olive pomace extract.