Prism 9.0 for macintosh

Prism 9.0 for Macintosh, Graphpad Software (San Diego, CA, USA), LLC, was used for all statistical computations. The results are given as means ± SEM. For multiple comparisons, repeated measurements one- and two-way ANOVA followed by Holm–Sidak post hoc test was used.

All statistical analyses were performed using Prism 9.0 for Macintosh (GraphPad Software). Unless stated otherwise, GMs and the upper 95% confidence intervals of GMs (between parentheses) were used as measures of central tendency. A nonparametric Mann-Whitney U test was used for the analysis comparing data from filaria-infected patients versus healthy donor volunteers. A Kruskal-Wallis 1-way ANOVA followed by Dunn’s multiple-comparison test was used to evaluate the statistical differences for the subset’s frequency, for the cytokine production by the different groups, and for the frequency of CD4⁺ T cells producing cytokines. Net production of cytokines (in pg/mL) and net frequency (percentage) of CD4⁺ T cells producing cytokines were calculated by subtracting the baseline level from the level following stimulation. The differences were considered statistically significant when the corrected P values were less than 0.05.