Log In Sign up
The largest database of trusted experimental protocols

Sc 514486

Manufactured by Novus Biologicals
Visit Supplier

Sc-514486 is a chemical compound that acts as a selective inhibitor of the IKK-2 enzyme, which is involved in the activation of the NF-κB signaling pathway. It is commonly used in cell-based and biochemical assays to study the role of NF-κB in various cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Goat anti rabbit 488, by Thermo Fisher Scientific (2 mentions) Tcs spe confocal microscope, by Leica camera (2 mentions) Ma5 14260, by Cell Signaling Technology (2 mentions) Ga508, by Agilent Technologies (2 mentions) Goat anti mouse 647, by Thermo Fisher Scientific (2 mentions) Tbx18, by Santa Cruz Biotechnology (2 mentions) Gata3, by Cell Signaling Technology (2 mentions) Vectashield, by Vector Laboratories (2 mentions)

2 protocols using sc 514486

1

Immunostaining Protocol for Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 4% paraformaldehyde for 15 min, washed with PBS (3 × 5min), and blocked in the presence of 0.1% Triton X-100 and 2% BSA for 1h at room temp. Cells were then incubated in primary antibody overnight, washed with PBS (3 × 5min), incubated with the secondary antibody in 2% BSA for 1hr, washed (4 × 10mins, adding 1mg/ml DAPI at the second wash), mounted using Vectashield (Vector Labs) and imaged using a Leica TCS-SPE confocal microscope at 10x,40x or 63x. Antibodies use in immunostaining were: TBX18 (Santa Cruz sc-514486, 1:200), Oct 4 (Novus Biologicals, NB11–1:150), p16 (Invitrogen MA5–14260, 1:250), p21 (Cell Signaling 2947S, 1:200), GATA3 (Cell signaling D13C9, 1:250), CGB (DAKO GA508; 1:200). Slides were rinsed with PBS-T and incubated with secondary antibodies: DAPI (0.02 µg/mL, Molecular Probes), goat anti-rabbit 488, and goat anti-mouse 647 (1:500, Molecular Probes) for 2 h at room temperature in blocking buffer. (EBdCas9 or NCdCas9 were visualized using endogenous mCherry expression) (SI Table S6).
Levy S., Somasundaram L., Raj I.X., Ic-Mex D., Phal A., Schmidt S., Ng W.I., Mar D., Decarreau J., Moss N., Alghadeer A., Honkanen H., Sarthy J., Vitanza N., Hawkins R.D., Mathieu J., Wang Y., Baker D., Bomsztyk K, & Ruohola-Baker H. (2022). dCas9 fusion to computer-designed PRC2 inhibitor reveals functional TATA box in distal promoter region. Cell reports, 38(9), 110457.
+ Open protocol
+ Expand
2

Immunostaining Protocol for Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 4% paraformaldehyde for 15 min, washed with PBS (3 × 5min), and blocked in the presence of 0.1% Triton X-100 and 2% BSA for 1h at room temp. Cells were then incubated in primary antibody overnight, washed with PBS (3 × 5min), incubated with the secondary antibody in 2% BSA for 1hr, washed (4 × 10mins, adding 1mg/ml DAPI at the second wash), mounted using Vectashield (Vector Labs) and imaged using a Leica TCS-SPE confocal microscope at 10x,40x or 63x. Antibodies use in immunostaining were: TBX18 (Santa Cruz sc-514486, 1:200), Oct 4 (Novus Biologicals, NB11–1:150), p16 (Invitrogen MA5–14260, 1:250), p21 (Cell Signaling 2947S, 1:200), GATA3 (Cell signaling D13C9, 1:250), CGB (DAKO GA508; 1:200). Slides were rinsed with PBS-T and incubated with secondary antibodies: DAPI (0.02 µg/mL, Molecular Probes), goat anti-rabbit 488, and goat anti-mouse 647 (1:500, Molecular Probes) for 2 h at room temperature in blocking buffer. (EBdCas9 or NCdCas9 were visualized using endogenous mCherry expression) (SI Table S6).
Levy S., Somasundaram L., Raj I.X., Ic-Mex D., Phal A., Schmidt S., Ng W.I., Mar D., Decarreau J., Moss N., Alghadeer A., Honkanen H., Sarthy J., Vitanza N., Hawkins R.D., Mathieu J., Wang Y., Baker D., Bomsztyk K, & Ruohola-Baker H. (2022). dCas9 fusion to computer-designed PRC2 inhibitor reveals functional TATA box in distal promoter region. Cell reports, 38(9), 110457.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!