The following fly stocks were obtained from Bloomington Drosophila Stock Center: w1118 (#3605), CS (#64349), UAS-mito-GFP (#8442), Elav-GS-Gal4 (#43642), Daughterless (DA)-GS-Gal4, Appl-Gal4 (#34040). Tsinghua Fly Center: Vimar RNAi (TH1142), Miro RNAi (TH2781), TRip background control (attp2, TB00072). We generated the following lines: hs-GluR1Lc, UAS-Vimar/tb, and UAS-Vimar/cyo. For all experiments, flies were kept at 25 °C with a 12:12 h light/ dark cycle and constant humidity (65%) on the standard sugar−yeast−agar medium (15 g/l agar, 50 g/l sugar, 100 g/l autolyzed yeast, 6 g/l nipagin and 3 ml/l propionic acid). Flies were raised at standard density in 100 ml bottles unless otherwise stated. All experiments of survival assay and behavior test were carried out with male and female files (10:10 in each tube). Female flies were used in all confocal microscopy imaging experiments.
Elav gs gal4
Manufactured by Bloomington Drosophila Stock Center
Elav-GS-GAL4 is a transgenic fly line that expresses the GAL4 transcription factor under the control of the elav promoter. The elav gene is neuron-specific, allowing for targeted gene expression in the nervous system of Drosophila.
Automatically generated - may contain errors
Lab products found in correlation
Uas mitogfp, by Bloomington Drosophila Stock Center (1 mentions)
Appl gal4, by Bloomington Drosophila Stock Center (1 mentions)
W1118, by Bloomington Drosophila Stock Center (1 mentions)
Gmr gal4, by Bloomington Drosophila Stock Center (1 mentions)
Trpl gal4, by Bloomington Drosophila Stock Center (1 mentions)
Mcherry rnai, by Bloomington Drosophila Stock Center (1 mentions)
Uas mcherry, by Bloomington Drosophila Stock Center (1 mentions)
Mhc gs gal4, by Bloomington Drosophila Stock Center (1 mentions)
4 protocols using elav gs gal4
1
Drosophila Genetics and Husbandry Protocol
2
Conditional Expression of Alzheimer's Genes in Drosophila
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UAS-Prosβ5 (21794) (70 (link)), UAS-hAPP.Abeta3-42 (64217), and Elav-GS-GAL4;UAS-hAPP;UAS-hBACE1 (56756) stocks were obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537). All lines were maintained on agar-cornmeal-dextrose-yeast growth medium (71 (link)) in a humidified 24°C incubator with 12-hour light/dark cycles. All crosses were set up with female virgins of the respective GAL4 driver line and male UAS-Prosβ5 or W1118 flies. Progeny were collected within 48 hours of eclosion and allowed to mate on 10% sugar/yeast (SY10) medium (72 (link)) for another 48 hours. Females were then separated and sorted into sets of 25 flies per vial containing SY10 medium supplemented with either 400 μM mifepristone (RU-486) or ethanol vehicle, mixed directly into the food. Blue dye #1 (8 μM) was added to food containing RU-486 for the purpose of identification. Carbon dioxide was used to briefly anesthetize flies for sorting. Flies were moved to vials of fresh medium every 2 to 3 days.
3
Drosophila Genotypes for Circadian Rhythm Research
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The genotypes of the Drosophila Melanogaster lines used in this study are listed in Supplementary Table 2 . The following lines were obtained from the Bloomington Drosophila Stock Center: Oregon R. (25125), GMR-GAL4 (1104), Elav-GS-GAL4 (43642), Trpl-GAL4 (52274), clkout (56754), UAS-csChrimson (55134), UAS-CLK-∆1 (36318), UAS-CLK-∆2 (36319), Gβ76c-RNAi (28507), tsp42Ej/sun-RNAi (29392), retinin-RNAi (57389), ATPα-RNAi (28073), nrv2-RNAi (28666), nrv3-RNAi (60367), and mCherry-RNAi (Bloomington RNAi-cnt, 35785). The following lines were obtained from the Vienna Drosophila Resource Center: arr1-RNAi (22196), RNAi-cnt (empty vector, 60100). The UAS-Clk line was a gift from Paul Hardin’s laboratory at Texas A&M University. The following lines were outcrossed to w1118 for this manuscript: UAS-CLK-∆1OC and Canton-SOC. The Trpl-GAL4 line was recombined with GAL80 for this manuscript: Trpl-GAL4; GAL80ts. Ethical approval was not requested for the experiments performed in this study given the exclusive use of fruit flies.
4
Drosophila Genetic Strains for Gene Expression
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Fly stocks used in this study were Act5c-GS-Gal4, WB-FB-GS-Gal4 (a combination of S32-GS-Gal4 and S106-GS-Gal4), and MHC-GS-Gal4, which were described in a previous study.6 (link) The elav-GS-Gal4 (#43642) and UAS-mCherry (#35787) were obtained from the Bloomington Drosophila Stock Center. The wild-type strains used were B3 and w1118. UAS-foxo fly stocks were also used.7 (link)
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