Paraffin-embedded sections of a colon tumor tissue array (T8235722–2, Biochain) were processed in a microwave (90 °C) with antigen-retrieval solution (pH 9) (S2367; Dako), treated with a peroxidase-blocking reagent, and then treated with a protein-blocking reagent (K130, X0909; Dako). The tissue sections were incubated with rabbit anti-EHMT2 antibody (68851; CST) followed by incubation with an HRP-conjugated secondary antibody (Dako). Immunoreactivity was visualized using a chromogenic substrate (Liquid DAB Chromogen; Dako). Finally, the tissue specimens were stained with Mayer’s hematoxylin solution (Hematoxylin QS; Vector Laboratories) for 5 s to discriminate the nucleus from the cytoplasm [22 (link)].
T8235722 2
Manufactured by BioChain
The T8235722–2 is a laboratory equipment designed for performing various scientific experiments and analyses. It serves as a core component in the research process, enabling researchers to conduct their work effectively. The product's detailed specifications and intended uses are not provided in this response, as an unbiased and factual description without extrapolation is requested.
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2 protocols using t8235722 2
1
Immunohistochemical Analysis of EHMT2 in Colon Tumor
2
Immunohistochemical Analysis of Colon Tumor Tissue
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Para n-embedded sections of colon tumor tissue array (T8235722-2, Biochain) were processed in a microwave (90℃) with antigen-retrieval solution (pH 9) (S2367; Dako), treated with a peroxidase-blocking reagent, and then treated with a protein-blocking reagent (K130, X0909; Dako). Tissue sections were incubated with rabbit anti-EHMT2 antibody (68851; CST) and rabbit anti-TNFAIP1 antibody (ab86934; abcam) followed by incubation with an HRP-conjugated secondary antibody (Dako). Immunoreactivitywas visualized with a chromogenic substrate (Liquid DAB Chromogen; Dako). Finally, tissue specimens were stained with Mayer's hematoxylin solution (Hematoxylin QS; Vector Laboratories) for 5 seconds to discriminate the nucleus from the cytoplasm.
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