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Ix71 invert fluorescence microscope

Manufactured by Olympus
Sourced in Japan

The IX71 is an inverted fluorescence microscope designed for a wide range of applications. It features a sturdy frame, a motorized stage, and a variety of illumination and imaging options to facilitate high-quality observations and image capture.

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2 protocols using ix71 invert fluorescence microscope

1

Detecting JHAMT in Drosophila Brain Cells

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For detecting JHAMT in the CA by immunohistochemistry, the brain-RG complexes were dissected from larvae at the EW stage. The Drosophila JHAMT rabbit polyclonal antibody (1:100) [13 (link)] and the FITC-conjugated Affinipure Goat Anti-Rabbit IgG secondary antibody (Jackson ImmunoResearch Inc.) were used, and the fluorescence signals were captured with an Olympus IX71 invert fluorescence microscope (Japan) [14 (link), 20 (link), 31 (link)].
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2

ROS Detection Using Fluorescence Microscopy

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The detection was performed by using a Reactive Oxygen Species Assay Kit (Applygen, Beijing, China). Briefly, cells pretreated with DCFH-DA (10 μM) at 37 °C for 10 min were sonicated and examined using an Olympus IX71 invert fluorescence microscope (Tokyo, Japan) after 20 min of incubation. The ROS production was calculated using the IPP software.
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