HEK 293T Flp-In T-Rex Cells (ThermoFisher, R78007) were cultured in Dulbecco’s modified eagle medium supplemented with 10% (v/v) fetal bovine serum, 20 mM HEPES pH 7.4, and GlutaMAX at 37 °C with 5% CO2. Cells were not authenticated, nor were they tested for mycoplasma contamination.
Hek293t flp in t rex cells
Manufactured by Thermo Fisher Scientific
HEK293T Flp-in T-rex cells are a stable mammalian cell line derived from human embryonic kidney cells. They are designed for inducible gene expression using the Flp-In T-REx system. The cells contain a single integrated FRT site and express the tetracycline repressor protein, allowing for tetracycline-inducible expression of genes of interest.
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3 protocols using hek293t flp in t rex cells
1
HEK 293T Flp-In T-Rex Cell Culture
2
Transient Transfection of HEK293T and C2C12 Cells
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HEK293T and C2C12 cells were obtained from the American Type Culture Collection (ATCC), which were used in more than 20 passages. HEK293T Flp-in T-rex cells were obtained from ThermoFisher Scientific (catalog no. R78007). The cell lines were frequently checked and tested for morphology and Mycoplasma contamination under a microscope but were not authenticated. All cell lines were cultured in high-glucose Dulbecco’s modified Eagle medium (DMEM) with 10% fetal bovine serum (FBS) at 37 °C in 5% CO2 (v/v). All cell lines were transiently transfected at 60–80% confluence using polyethyleneimine.
3
Cell Line Maintenance and Transfection
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HEK293T and C2C12 cells were obtained from the American Type Culture Collection (Manassas, VA, USA), which were used at >20 passages. HEK293T Flp-in T-rex cells were obtained from Thermo Fisher Scientific (Cat. no. R78007). The cell lines were frequently checked and tested for morphology and mycoplasma contamination under a microscope but were not authenticated. All cell lines were maintained in high-glucose Dulbecco's modified Eagle medium (DMEM) with 10% fetal bovine serum (FBS) at 37°C in 5% CO2 (v/v).
All cell lines were transiently transfected at 60-80% confluence using polyethyleneimine (PEI).
All cell lines were transiently transfected at 60-80% confluence using polyethyleneimine (PEI).
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