Mouse monoclonal antibody against

Western blot analysis was performed as previously described [6 (link)]. For Western blot analysis of phospho-p53, we added phosphatase inhibitors in lysis buffer (PhosSTOP, Roche). The primary antibodies were: rabbit polyclonal antibody against SOX14 (Abcam, Cambridge, UK, ab149047, diluted 1:400), mouse monoclonal anti-α-Tubulin (Calbiochem, MA, USA, CP06, diluted 1:30000), mouse monoclonal antibody against GAPDH (Abcam, Cambridge, UK, ab9484, diluted 1:5000), mouse monoclonal antibody against cleaved PARP (Cell Signaling, Technology, Danvers, MA, USA, Asp214, diluted 1:1000), mouse monoclonal antibody against p53 (DO-1) (Santa Cruz Biotechnology, Texas, USA, sc-126X, diluted 1:1000), rabbit monoclonal antibody against p21^Waf1/Cip1 (Cell Signaling, Technology, Danvers, MA, USA, 2947, diluted 1:1000) and rabbit antibody against phospho-p53 (Cell Signaling, Technology, Danvers, MA, USA, diluted 1:1000). Secondary antibodies used for Western blot were: horseradish peroxidase-conjugated anti-mouse and anti-rabbit IgG (Amersham Biosciences, NJ, USA, diluted 1:10000 or Active motif, La Hulpe, Belgium, 1:5000). Immunoreactive bands were detected by chemiluminescence (Immobilion substrate, Millipore, MA, USA). The density of protein bands on Western blots was quantified using ImageJ software.

RD was purchased from Guangzhou Honsea Industry Co. Ltd. Rabbit monoclonal antibodies against phospho-Ser32 IκBα (#2859), p65 (#8242), phospho-Ser536 p65 (#3033), β-actin (#4970) and a mouse monoclonal antibody against IκBα (#4814) were purchased from Cell Signaling Technology (Cell Signaling, USA). Goat polyclonal secondary antibodies against mouse (#A0216) or rabbit (#A0208) were purchased from Beyotime Biotechnology (Beyotime, China).