Horseradish peroxidase conjugated anti mouse and anti rabbit secondary antibodies

Manufactured by Promega

Horseradish peroxidase conjugated anti-mouse and anti-rabbit secondary antibodies are laboratory reagents used in immunoassay techniques. They are designed to bind to primary antibodies raised in mouse or rabbit, and the attached horseradish peroxidase enzyme can be used for signal detection.

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Horseradish peroxidase (HRP) conjugated secondary anti-mouse and anti-rabbit antibodies Anti-rabbit peroxidase-conjugated secondary antibodies Secondary horseradish peroxidase conjugated antibodies Anti-mouse secondary antibodies Horseradish peroxidase

2 protocols using horseradish peroxidase conjugated anti mouse and anti rabbit secondary antibodies

Antibody-based Protein Quantification Assay

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Antibody against ESM1 (ab103590) was purchased from Abcam (Cambridge. MA). Antibodies against p21 (sc-397; 1:1000), MMP-2 (sc-53630; 1:1000), MMP-9 (sc-6840; 1:1000), uPA (sc-14019; 1:1000), cyclin D1 (sc717; 1:1000), and β-actin (sc-47778; 1:2000) were purchased from Santa Cruz Biotechnology (Dallas, Texas). Horseradish peroxidase conjugated anti-mouse and anti-rabbit secondary antibodies were obtained from Promega (Madison, WI). MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] was purchased from Sigma (St. Louis, MO). Recombinant Human TIMP-1 Protein was purchased from R&D Systems, Inc (Minneapolis, MN). Antibody against TIMP-1(#8946; 1:1000) was purchased from Cell Signaling Technology Inc. (Beverly, MA, USA).

Chen C.M., Lin C.L., Chiou H.L., Hsieh S.C., Lin C.L., Cheng C.W., Hung C.H., Tsai J.P, & Hsieh Y.H. (2017). Loss of endothelial cell-specific molecule 1 promotes the tumorigenicity and metastasis of prostate cancer cells through regulation of the TIMP-1/MMP-9 expression. Oncotarget, 8(8), 13886-13897.

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Signaling Pathway Regulation in Cancer

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The antibodies against EMP3, β-actin, p21, p27, SKP2, cyclin E, cyclin D1, MMP-9, uPA, p-ERK1/2, ERK1/2, p-Akt (Ser473) Akt, p85 subunit of PI3K, p110 subunit of PI3K, Ki-67, siRNAs against mock (neo), Akt (siAkt), and (siERK) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Horseradish peroxidase-conjugated anti-mouse and anti-rabbit secondary antibodies were obtained from Promega (Madison, WI). MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide], propidium iodide (PI), and DAPI (4′-6-diamidino-2-phenylindole) were purchased from Sigma (St. Louis, MO). PD98059 and LY294002 were bought from Calbiochem (San Diego, CA).

Hsieh Y.H., Hsieh S.C., Lee C.H., Yang S.F., Cheng C.W., Tang M.J., Lin C.L., Lin C.L, & Chou R.H. (2015). Targeting EMP3 suppresses proliferation and invasion of hepatocellular carcinoma cells through inactivation of PI3K/Akt pathway. Oncotarget, 6(33), 34859-34874.

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