Renilla tk plasmid

Manufactured by Promega

The Renilla-TK plasmid is a laboratory tool used to express the Renilla luciferase reporter gene. It contains the Renilla luciferase coding sequence and the thymidine kinase (TK) promoter, which drives the expression of the reporter gene.

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Lab products found in correlation

Dual luciferase reporter assay kit, by Promega (2 mentions)

Close spelling variants of this product

Renilla luciferase expression of pRL-TK plasmids P-TK Renilla plasmid PRL‐TK Renilla reference plasmid PRL-TK Renilla plasmid PRL-TK Renilla luciferase plasmid PRL-TK™ Renilla reniformis luciferase plasmid PRL-TK Renilla luciferase reporter plasmid TK-Renilla luciferase plasmid TK-Renilla reporter plasmid Renilla luciferase-thymidine kinase (pRL-TK) plasmid

2 protocols using renilla tk plasmid

Regulation of miR-204-5p Promoter Activity

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To determine the influence of Hdac2 interactions with Sp1 on the promoter activity of miR-204-5p, HEI-OC1 cells were seeded into 96 wells (4 × 10³ cells per well) and then co-transfected with one of the specific luciferase reporter plasmids, siRNAs, miR-204-5p inhibitor, or expression vectors, and Renilla-TK plasmid (Promega) transfection reagent. After incubation for 48 h, luciferase activities were examined with the Dual-Luciferase Reporter Assay kit (Promega, Madison, WI, USA) according to the manufacturer. Luciferase activities were normalized by Renilla luciferase activities. The data were expressed as fold changes relative to corresponding control group.

Xie L., Zhou Q., Chen X., Du X., Liu Z., Fei B., Hou J., Dai Y, & She W. (2021). Elucidation of the Hdac2/Sp1/miR-204-5p/Bcl-2 axis as a modulator of cochlear apoptosis via in vivo/in vitro models of acute hearing loss. Molecular Therapy. Nucleic Acids, 23, 1093-1109.

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Luciferase Reporting of miR-200b Activity

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SPC-A1/DTX or H1299/DTX cells (4×10³/well) were seeded into 96-well plates and cotransfected with luciferase reporter plasmids and sh-RNAs with (or without) miR-200b inhibitors. The Renilla-TK plasmid (Promega) was cotransfected into all samples. After 48 h, luciferase activities were measured with the Dual-Luciferase Reporter Assay kit (Promega). The relative luciferase activities were normalized by Renilla luciferase activities. The data were relative to the fold change of the corresponding control groups defined as 1.0.

Chen D.Q., Pan B.Z., Huang J.Y., Zhang K., Cui S.Y., De W., Wang R, & Chen L.B. (2014). HDAC 1/4-mediated silencing of microRNA-200b promotes chemoresistance in human lung adenocarcinoma cells. Oncotarget, 5(10), 3333-3349.

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