The Washington University Animal Care and Use Committee approved this study. All mice were purchased from the Jackson laboratory (Bar Harbor, ME, USA) and housed in groups in individually ventilated cages (5 mice/cage). All animals had ad libitum access to low-fluorescence mouse chow and water and a 12:12 h light:dark cycle. In this experiment, 10-wk-old male C57BL/6J mice were used. Under anesthesia, the right knees were kept in a flexed position. Infrared FluoroSphere (715/755)-loaded PEG hydrogel microspheres (15 μL per joint) with or without PRP (n = 3) were injected intra-articularly on day 0 with a 30-gauge needle. The fluorescent signal was followed up to 42 days after injection by an In Vivo Imaging System (Xenogen In Vivo Imaging System 50, Perkin Elmer). Mice receiving PBS injection served as control.
Xenogen in vivo imaging system 50
Manufactured by PerkinElmer
The Xenogen In Vivo Imaging System 50 is a high-performance, non-invasive imaging system designed for pre-clinical research applications. The system is capable of detecting and quantifying bioluminescent and fluorescent signals from living animals.
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2 protocols using xenogen in vivo imaging system 50
1
Intra-articular Hydrogel Microspheres and PRP in Mice
2
Bioluminescence Imaging of Tumor Growth
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To monitor tumor growth, bioluminescence imaging was performed on the indicated days using the Xenogen In Vivo Imaging System 50 (IVIS; Perkin Elmer) as previously described and analyzed using Living Image analysis software (Perkin Elmer).20 (link) Briefly, mice were injected with D-luciferin i.p. (150 mg/kg) and anesthetized with isoflurane 10 min before imaging.
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