Fluorescein isothiocyanate fitc dextran 40 kda

Dulbecco’s modified Eagle's medium (DMEM), penicillin and streptomycin were obtained from Corning Cellgro (Manassas, VA, USA). Fetal bovine serum (FBS) was obtained from Gibco-BRL (Life Technologies, Grand Island, NY, USA). Amantadine HCl (A1260; Molecular Weight: 187.7), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), PI, Triton X-100, ribonuclease A (RNase A), DTX, normal melting point agarose, low melting point agarose and fluorescein isothiocyanate (FITC)-dextran (40 kDa) were obtained from Sigma (St. Louis, MO, USA). Caspase-Glo 3/7 assay kit was purchased from Promega (Madison, WI, USA). Alexa Fluor 488 Annexin V/Dead Cell Apoptosis kit and Click-iT EdU Alexa Fluor 488 flow cytometry assay kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). CFSE cell-division tracker kit was obtained from BioLegend (San Diego, CA, USA).

Commercially available RE was purchased from Vitiva (Markovci, Slovenia). CA and CL were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Acetonitrile and formic acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Butyl paraben was purchased from Acros Organics (Fair Lawn, NJ, USA). Mouse plasma was purchased from Innovative Research Inc. (Novi, MI, USA). Dextran sodium sulfate was purchased from Alfa Aesar (Haverhill, MA, USA). RNAlater was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fluorescein isothiocyanate (FITC)–dextran (40 kDa) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Sestrin 2, zonula occludens-1 (ZO-1), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rabbit polyclonal primary antibodies were purchased from Proteintech (Rosemont, IL, USA). Horseradish peroxidase (HRP)-linked anti-rabbit secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).