On the seventh day after surgery and treatment, 300 μL of cerebrospinal fluid (CSF) from each rat and 1.2 mL of fresh blood were collected. After centrifugation, the CSF samples and serum samples were added into plates to measure the concentration of IL-6, IL-8 and TNF-α using a Rat IL-6 enzyme-linked immunosorbent assay (ELISA) Kit (Beyotime, China), Rat IL-8 ELISA Kit (NeoScientific, United Kingdom) and Rat TNF-α ELISA Kit (Beyotime, China), respectively, in accordance with the manufacturers’ guidelines.
Rat il 6 enzyme linked immunosorbent assay elisa kit
Manufactured by Beyotime
Sourced in China
The Rat IL-6 Enzyme-Linked Immunosorbent Assay (ELISA) Kit is a quantitative analytical tool used to measure the concentration of Interleukin-6 (IL-6) in rat biological samples. The kit utilizes the ELISA technique to detect and quantify the target analyte.
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Anti stat3 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti bcl 2 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti caspase 3 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti p jak2 antibody, by Abcam (1 mentions)
Ag490, by Merck Group (1 mentions)
Anti β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Anti jak2 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti p stat3 antibody, by Santa Cruz Biotechnology (1 mentions)
Lactate dehydrogenase ldh cytotoxicity assay kit, by Beyotime (1 mentions)
2 protocols using rat il 6 enzyme linked immunosorbent assay elisa kit
1
Cytokine Analysis of Post-Surgical CSF and Serum
2
Eriodictyol Inhibits Inflammatory Pathways
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Eriodictyol (EDT) (≥95.0%, Cat No. 89061), AG 490 (ak-2 protein tyrosine kinase inhibitor, Cat No. T3434) and 2,3,5-Triphenyltetrazolium chloride (TTC, ≥95.0%, Cat No. T8877), were purchased from Sigma-Aldrich (Shanghai, China). Lactate dehydrogenase (LDH) Cytotoxicity Assay Kit (Cat No. C0017), Rat IL-6 Enzyme-Linked ImmunoSorbent Assay (ELISA) Kit (Cat No. PI328), Rat TNF-α ELISA Kit (Cat No. PT516) and Colorimetric TUNEL Apoptosis Assay Kit (Cat No. C1098) were purchased from Beyotime Biotechnology (Shanghai, China). Creatine Kinase Activity Assay Kit (Cat No. BC1140) Rat CRP EasyTestTM ELISA kit (Cat No. 1030026) were purchased from Beijing Solarbio Science & Technology Co., Ltd., Rat IL-8 ELISA Kit (Cat No. BP-E30583) was purchased from Shanghai Langton Biotechnology Co., Ltd., Anti-Bax antibody (Cat No. sc-7480), Anti-Bcl-2 antibody (Cat No. sc-7382), Anti-Caspase-3 antibody (Cat No. sc-56053), Anti-JAK2 antibody (Cat No. sc-390539), Anti-STAT3 antibody (Cat No. sc-8019), Anti-p-STAT3 antibody (Cat No. sc-8059) and anti-β-actin antibody (Cat No. sc-47778) were all purchased from Santa Cruz Biotechnology. Anti-p-JAK2 antibody (Cat No. ab32101) was purchased from Abcam (Shanghai, China).
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