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Diphenyl 1 pyrenylphosphine

Manufactured by Dojindo Laboratories
Sourced in Japan

Diphenyl-1-pyrenylphosphine is a chemical compound used as a fluorescent labeling reagent. It is a phosphine-based compound that can be used to label biomolecules, such as proteins or DNA, with a pyrene fluorophore. The pyrene moiety provides a distinctive fluorescent signal that can be detected and analyzed using specialized analytical techniques.

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2 protocols using diphenyl 1 pyrenylphosphine

1

Cell Viability and Oxidative Stress Assay Protocol

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The Cell Counting Kit-8 and Diphenyl-1-pyrenylphosphine (DPPP) were purchased from Dojindo (Tokyo, Japan), anti-monocarboxylate transporter 1 (MCT1) antibodies were obtained from Alpha Diagnostic International, Inc. (San Antonio, TX), and anti-caspase 9 antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA). Radioisotope of [2-14C]-Acetic acid sodium salt was purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO). Acetic acid, hydrochloric acid and N-Acetyl-l-cysteine (NAC) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The 12% Bis Tris gels were obtained from Life Technologies Japan, Ltd. (Tokyo, Japan), and the Can Get Signal reagents were obtained from TOYOBO Co., Ltd. (Osaka, Japan). 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) was purchased from LABOTEC Co., Ltd. (Tokyo, Japan).
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2

Mitochondrial Redox Modulation Protocol

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Risedronate was obtained from LKT Laboratories, Inc. (St. Paul, MN). QD powder was purchased from Seishinshouakudo (Tokyo, Japan), which imports QD from China. Cell Counting Kit-8 (CCK-8), diphenyl-1-pyrenylphosphine (DPPP), and MitoRed were purchased from Dojindo (Kumamoto, Japan). 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was purchased from LABOTEC Co., Ltd. (Tokyo, Japan) and β-nicotinamide adenine dinucleotide (NADH), d-glutamic acid, and succinic acid were purchased from Sigma-Aldrich (St. Louis, MO). Malic acid, indirubin, and indigo were purchased from Wako Pure Chem. Ind., Ltd. (Osaka, Japan) and MitoTracker Green FM was purchased from Cell Signaling Technology Japan, K.K. (Tokyo, Japan).
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